Secretory Pathway Flashcards
SRP does 2 things
Stops translation
Docks with ER membrane
Types 1,2,and 3 are all
cotranslated
Type 1
N term in ER lumen
Type 2
Has COO in ER lumen
Explain pathology of CF
When CFTR is defected or absent, a lot more sodium goes in the cell and also a lot more water. This results in dehydration the cilia layer and messes up the bicarb layer giving thick mucous and thin water layer.
CFTR allows chloride to do what
Cl- to go out, which allows sodium to go in.
Apoptosis (phosphatidyl serine)
Gets flipped to to outer membrane to undergo apoptosis.
Cytochromes do what?
oxidize—-> water soluble—->excreted
SRP does two things
- Stops translocation
- Docs with ER membrane.
**When signal sequence gets into channel, it opens the channel and prevents any calcium escape.
N term on outside or ER lumen
Type 1
C term on outside or ER lumen
Type 2
Which type has a signal sequence cleaved off
Type 1
What does it mean if glucose is on a protein ?
Protein aint folded right
Erp57 .. what’s it do?
Recognizes if a protein has formed disulfide bonds correctly.
**There should be NO FREE disulfides in a protein sitting in the ER.
Glucosyl transferase
Recognizes if a protein is folded or not by looking for a hydrophobic patch on the inside.
It then decides whether or not to put another glucose on it.
See patch = put glucose
Don’t see patch = do nothing.
