Secondary Hemostasis

Question 1

Plasma Coagulation factors
1. aka
2. inactive form
3. generates _____
4. substances upon which enzymes act upon

Answer 1

1. soluble plasma coagulation factors
2. zymogen
3. thrombin
4. substrates

Question 2

Plasma Coagulation Factors
I = ____ (biologic half life)
II = ____ (biologic half life)
III = ____ (remarks)
IV = ____ (remarks)
V = ____ (biologic half life)
VII = ____ (biologic half life)

Answer 2

Fibrinogen = Thrombin substrate (2-4 days)
Prothrombin = Serine protease (3-4 days)
Tissue Factor = Cofactor (insoluble)
Calcium = Mineral (ionized state )
Proaccelerin = Cofactor (36hrs)
Proconvertin = Serine protease (3-6hrs)

Question 3

Plasma Coagulation Factors
VIII:C = ____ (biologic half life)
IX = ____ (biologic half life)
X = ____ (biologic half life) + remarks
XI = ____ (biologic half life)
XII = ____ (biologic half life) + remarks
XIII = ____ (biologic half life)

Answer 3

Antihemophilic Factor = Cofactor (10-14hrs)
Plasma Thromboplastin Component = Serine protease (18-24hrs)
Stuart-Prower Factor = Serine protease (40-60hrs) + 2mons at 4C
Plasma Thromboplastin Antecedent = Serine protease (40-70hrs)
Hageman Factor = Serine protease (50-70hrs) + 4C for 3mons
Fibrin Stabilizing Factor = Thrombin Substrate (11-14 days)

Question 4

Plasma Coagulation Factors
Fletcher factor = ____ (biologic half life)
Fitzgerald factor = ____ (biologic half life)
VWF = ____ (biologic half life)
PF3 = ____ (remarks)

Answer 4

PK = Serine protease (35hrs)
HMWK = Cofactor (9-10hrs)
VWF = Factor VIII carrier; (24hrs)
PF3 = Assembly molecule (released by platelets)

Question 5

Nomenclature for Factor VIII of the International Committee on Thrombosis & Hemostasis

1. molecule as it it circulates in plasma
   - composed of ___ & ___ portions noncovalently
2. portion of molecule responsible for binding to endothelium and supporting normal platelet adhesion and function
   - tested by _____
3. acting in intrinsic system as cofactor at IXa (w/ calcium) in the conversion of factor X to Xa
   - tested by
4. antigenic property of procoagulant portion
   - measured by
5. antigenic portion of protein portion of vWF
   - measured by ____
6. ristocetin cofactor activity
   - factor ____-related

Answer 5

1. VIII/vWF
   - VIII:C & VIII:vWF
2. VIII:vWF
   - bleeding time
3. VIII:C
   - partial thromboplastin time
4. VIIIC:Ag
   - immunologic monoclonal antibody techniques
5. VIIIR:Ag
   - immunologic techniques of Laurell Rocket
6. VIIIR:RCo
   - factor VIII-related activity

Question 6

Groups of Coagulation Factors
1. Fibrinogen group
2. Prothrombin group
- proteins
3. Contact group

Answer 6

1. I,V,VIII,XIII
2. II,VII,IX,X
   - S,C,Z (Regulatory Proteins)
3. XI,XII,PK,HMWK

Question 7

Coagulation Cascade: Coagulation Complexes
1. Intrinsic Tenase Complex
2. Extrinsic Tenase Complex
3. Prothrombinase Complex

Answer 7

1. Factor VIIIa, Factor IXa, Calcium ions, & Phospholipids
2. Factor VIIa, Tissue Factor, & Calcium ions
3. Factor Xa, Factor Va, Calcium ions & Phospholipids

Question 8

Other Related Systems
1. involves chemotaxis, pain sensation, and mediate inflammatory responses
2. kallikrein, XIIa, & HMWK act as plasminogen activator
3. mediate immune & allergic responses

Answer 8

1. Kinin System
2. Fibrinolytic System
3. Complement System