4

Question 1

Direct Method:

1. ____ is using Rees & Ecker diluting fluid
2. reference method for platelet count
   - example
   - diligent fluid
   - counting chamber

Indirect Method
1. this can be used in Estimation and not only in Count
- diluent

Answer 1

1. Tocantins Method
2. Phase Contrast
   - Brecker-Cronkite Method
   - 1% ammonium oxalate
   - Sprite-Briteline #1475
3. Fonlo’s method
   - 14% Magnesium sulfate

Question 2

normal in platelet estimation

Answer 2

200k-400k

Question 3

methods under bleeding time
1. puncture is done in earlobe
2. puncture is done in forearm
3. template containing standardized slit
- ref value
4. finger puncture

Answer 3

1. Duke method
2. Ivy method
3. Template BT (by Mielke)
4. Copley-Lalitch

Question 4

_____: to know if platelets can adhere in vitro

1. ref value
2. this collects 2 whole blood samples
3. this collects genius and capillary

Answer 4

Retention Test
1. 26-60%
2. Glass Bead Method
3. Borshgervinct Method

Question 5

_____: gold standard for evaluation of aspirin resistance
1. give 3 aggregating agents
2. reading of aggregometer response
- light transmittance aggregometer
- electrical impedance
- simultaneous measurement of aggregation and secretion
- thru ____, it can measure these secretions- chemiluminescence

Answer 5

Platelet Aggregation Test
1. thrombin, ADP, collagen
2.
- Platelet-Rich Plasma Aggregometry
- Whole-Blood Platelet Aggregometry
- Optical Lumi -Aggregometer

Question 6

Clot Refraction Time
1. clot refraction begins within ____ and complete within ____
2. give 2 normal clot refraction requirement
3. contractile protein which helps platelet to contract
4. methods
- quali test for presence or absence of retraction; castor oil method
- test tube method
- quanti tig ate estimate of degree of refraction; most common

Answer 6

1. 30mins; 24hrs
2. # of functioning platelets, fibrinogen
3. Thrombosthenin
4. methods
   - Hirschboek Method
   - Stefanini Method
   - Macfarlane Method

Question 7

Plasma Coagulation Factors
1. Aka
2. Inactive form
3. generates ____

Classification of Coag Factors
1. this enzymes accelerates the next enzyme
2. this enzyme transfers another amino acid to enzyme

Answer 7

Plasma Coagulation Factors
1. Soluble Plasma Coagulation Factors
2. Zymogens
3. Thrombin

Classification of Coag Factors
1. Serine protease
2. Transaminase

Question 8

Preferred name

I
II
III
IV
V
VII

Function
I
II
III
IV
V
VII

Answer 8

Preferred name

Fibrinogen
Prothrombin
Tissue factor
Calcium
Proaccelerin
Proconvertin

Function
Thrombin substrate
Serine protease
Cofactor
Mineral
Cofactor
Serine protease

Question 9

Preferred name

VIII:C
IX
X
XI
XII
XIII

Function
VIII:C
IX
X
XI
XII
XIII

Answer 9

Preferred name

Antihemophilic Factor
Plasma Thromboplastin Component
Stuart-Prower Factor
Plasma Thromboplastin Antecedent
Hageman Factor
Fibrin Stabiliziling Factor

Function
Cofactor
Serine protease
Serine protease
Serine protease
Serine protease
Thrombin substrate of Fibrinogen

Question 10

1. Fletcher factor
   - fxn
2. Fitzgerald factor
   - fxn

Other procoagulants
1. _____ =
2. _____ =

Answer 10

1. Prekallikrein
   - serine protease
2. High Molecular Weight Kininogen
   - Cofactor

Other procoagulants
1. VWF = Factor VIII carrier
2. Platelet Factor 3 = assembly molecule