SDS PAGE Flashcards
1
Q
Agarose Gel Electrophoresis
A
DNA or RNA separation
TAE or TBE for DNA
MOPS or formaldehyde for RNA
2
Q
PAGE
A
Polyacrylamide Gel Electrophoresis
Nucleus acids 5–500bp
Proteins 10-230kDa
Smaller things move faster/farther
3
Q
SDS
A
Linearizes protein
Coats protein with negative ions so migration is directly proportional to size
4
Q
Discontinuous buffer system
A
Stacking gel, resolving gel, and tank buffers different pH and ionic strength
Protein move quickly through stacking gel, slower through resolving gel
