Science - Biology - Cell Structures - B1 Flashcards
roughly, what size are animal cells?
vary from 0.01mm-0.05mm
roughly, what size are plant cells?
vary from 0.01mm-0.1mm
what is the job of a microscope?
magnify image of biological specimen so it appears larger
what type of microscope is generally used in schools?
compound microscope
name 2 lenses compound microscopes use to magnify specimen?
eyepiece and objective lens
how do you calculate magnification of a microscope?
magnification of eyepiece x magnification of objective
If magnification of eyepiece is x10, and magnification of objective is x40, what is magnification of microscope?
x400
what is the best unit to measure most cells?
micrometere(μm)
for sub-cellular structures, such as ribosomes, what is the best unit of measurement?
nanometre (nm)
describe the method of how to prepare a biological cell for examination in animal cheek cells
- put a small drop of water on microscope slide
- gently swab inside of your mouth with a clean cotton bud
-gently rub the cotton bud in the drop of water, you will be able to see the cells with the naked eye
describe the method on how to prepare a biological cell for examination in plant cells - Onion epidermal cells
- put a small drop of water on microscope slide
- peel some onion skin from inside one of the leaves in an onion bulb
- use forceps to transfer the drop of water, make sure onion skin is flat, and there is no trapped air bubbles. Stain cells with iodine
describe the method on how to prepare a biological cell for examination - human hair or animal fur
- put a small drop of glycerol on microscope slide
- cut a small piece of hair or fur
- use forceps to transfer to a drop of glycerol
- place a small square or circle of thin glass called a coverslip over the specimen
- put a drop of the stain on the slide, next to the coverslip
- draw the stain under the coverslip by placing a piece of filter paper next to it
why is a coverslip needed for examining cells ?
it protects the microscope and prevents the slide from drying out when it’s being examined
what is a mounted needle used for?
holding specimen in place as coverslip is lowered
why are stains used?
stains are used to add contrast as most cells are colourless,certain stains are also used to stain specific cell structures or cell products
name 3 risks when using a microscope
-care must be taken when looking down the microscope if the illumination is too bright.
-care when using microscope stains.
-care when handling coverslips, microscope slides and mounted needles.
describe the method on how to use a microscope
- rotate the objective lenses so that the low power, eg x10, is in line with the stage
- turn the coarse focus so that the stage is as close to the objective lens as possible. You should not look through the microscope to do this
- place the microscope slide – either one you have prepared, or a permanent slide – on the stage. Line it up so that the specimen – if you can see it – is in the centre of the stage, where the light passes through
- focus the slide towards you by turning the coarse focus adjustment
- draw a low power image or record a digital image of what you see. Then, rotate the objectives so that the high power objective, eg x40, is in line with the stage
- bring the slide back into focus using the fine focus adjustment. If you do not succeed, go back to low power and re-focus, then try again
why is a low power diagram used when examining cells?
-as a plan to show the arrangement of any distinct regions of the tissue, for example the tissues in a plant root
-to show the outline of individual cells that make up the tissue, if the tissue is uniform
why is a high power diagram used when examining cells?
a detailed image of a part of the slide. It is usually drawn to show a single cell, eg of a single cheek cell or onion cell
describe the microscope in the 1590s
dutch spectacle makers Janssen experimented with putting lenses in tubes. they made the first compound microscope. None of their microscopes have survived, but they are thought to have magnified from ×3 to ×9
describe the microscope in 1650
robert hooke - observed and drew cells using a compound microscope.
describe the microscope in the late 1600s
dutch scientist Antonie van Leeuwenhoek constructed a microscope with a single spherical lens. it magnified up to ×275
describe the microscope in the 1800s
the optical quality of lenses increased and the microscopes are similar to the ones we use today
what is the maximum magnification using a light microscope?
x1500
name a limit of the light microscope
resolution
what do electron microscopes use instead of light rays?
a beam of electrons
name 2 types of electron microscope
- scanning electron microscope (SEM)
- transmission electron microscope (TEM)
give a disadvantage of the electron microscope
it’s expensive
what is cytoplasm ?
a jelly-like material that contains dissolved nutrients and salts and structures called organelles. it is where many of the chemical reactions happen
what is the function of the nucleus?
contains genetic material, including DNA, which controls the cell’s activities
what is the function of cell membrane?
its structure is permeable to some substances but not to others. it therefore controls the movement of substances in and out of the cell
what is the function of mitochondria ?
organelles that contain the enzymes for respiration, and where most energy is released in respiration
what is the function of ribosomes ?
tiny structures where protein synthesis occur
what 3 additional structures to plant cells have that animal cells don’t ?
- cell wall
- permanent vacuole
- chloroplasts
what is the function of the cell wall ?
made from cellulose fibres and strengthens the cell and supports the plant
what is the function of the chloroplasts ?
organelles that contains the green pigment, chlorophyll, which absorbs light energy for photosynthesis. contains the enzymes needed for photosynthesis
what is the function of the vacuole ?
filled with cell sap to help keep the cell turgid
how can cell size be measured ?
using an eyepiece graticule
what are prokaryotic cells ?
a simple cell that does not have a nucleus - the DNA is free in the cytoplasm
what are cells of bacteria called ?
prokaryotic cells
what are eukaryotic cells?
a type of cell that has a nucleus
what are cells of plants, animals, and fungi called ?
eukaryotic cells
what size is a eukaryotic cell ?
most are 5 μm – 100 μm
what size is a prokaryotic cell ?
most are 0.2 μm – 2.0 μm
name outer layers of the eukaryotic cell
cell membrane - surrounded by cell wall in plants and fungi
name outer layers of the prokaryotic cell
cell membrane - surrounded by cell wall
what is the cell contents of a eukaryotic cell ?
cytoplasm, cell organelles include mitochondria, chloroplasts in plants and ribosomes
what is the cell contents of a prokaryotic cell ?
cytoplasm, ribosomes, no mitochondria or chloroplasts
where is the genetic material in a eukaryotic cell ?
DNA in a nucleus - plasmids are found in a few simple eukaryotic organisms
where is genetic material in a prokaryotic cell ?
DNA is a single molecule, found free in the cytoplasm - additional DNA is found on one or more rings called plasmids
what type of cell division do eukaryotic cells use ?
mitosis
what type of cell division do prokaryotic cells use ?
binary fission
what is a specialised cell ?
specialised cells are cells designed to carry out a particular role in the body
name specialised functions of a sperm cell
- head of the sperm contains the genetic material for fertilisation
- acrosome in the head contains enzymes so that the sperm can penetrate an egg
- middle piece is packed with mitochondria to release energy needed to swim and fertilise the egg
- tail enables sperm to swim
name specialised functions in a nerve cell
- nerve cell is extended, so that nerves can run to and from different parts of the body to the central nervous system
- cell has extensions and branches, so that it can communicate with other nerve cells, muscles and glands
- nerve cell is covered with a fatty sheath, which insulates the nerve cell and speeds up the nerve impulse
name specialised functions in a muscle cell
- muscle cells contain filaments of protein that slide over each other to cause muscle contraction
- well-developed mitochondria to provide the energy for muscle contraction
name specialised functions in a root hair cell
- large surface area to provide contact with soil water
- thin walls so as not to restrict the movement of water
name specialised functions in a xylem cell
- no top and bottom walls between xylem vessels, so there is a continuous column of water running through them
- walls become thickened and woody. They therefore support the plant
what do chromosomes carry?
genetic information in a molecule called DNA
where are chromosomes found?
in the nucleus
what is DNA ?
material inside the nucleus of cells, carrying the genetic information of a living being
where is DNA ?
in the nucleus, within chromosomes
what is archaea ?
microorganisms which are similar in size and structure to bacteria but are genetically different
what is a biological specimen ?
in microscopy, a whole organism, cells, tissues, a small piece of tissue, or other structures, eg hair, pollen grains, that are viewed with a microscope
what does calibrate mean ?
to set an instrument or scale against a standard
what is a compound microscope ?
compound microscope uses two lenses, the objective lens and the eyepiece. The very short focal length objective lens produces a greatly-magnified image, then the short focal length eyepiece magnifies this further
what is a eukaryotic cell ?
a type of cell that has a nucleus
what is an eyepiece ?
the lens at the top of a compound microscope. It has a longer focal length than the objective lens and magnifies the image produced by the objective
what is field of view ?
the area seen when looking through a microscope
what is a graticule ?
a glass or plastic disc fitted into the eyepiece of a microscope. The graticule has a scale ruled on it and is used to estimate the size of a specimen when viewed with a microscope
what is a light microscope ?
device that uses visible light and a series of lenses to produce an enlarged image of an object
what is magnification ?
the amount that an image of something is scaled up when viewed through a microscope
what is a micrograph ?
a photograph taken of a microscopical image
what is mitochondria ?
structures in the cytoplasm of all cells where aerobic respiration takes place
what is a mounted needle ?
a needle mounted in a wooden or metal handle for use in microscope work or dissection
what is an objective lens ?
the lens near the specimen being examined - which magnifies the image at different levels
what is order of magnitude ?
a number, to the base ten, often used to make comparisons. For each order of magnitude, a number is ten times the previous one
what is a prokaryotic cell
a simple cell that does not have a nucleus - DNA is free in cytoplasm
what is resolution ?
the fineness of detail that can be seen in an image - the higher the resolution of an image, the more detail it holds. In computing terms, resolution is measured in dots per inch
what happens in the ribosome ?
the site of protein synthesis
what is a stage micrometre ?
a glass slide with a scale etched on it. It is used to calibrate the eyepiece graticule of a microscope
what does sub-cellular mean ?
structures smaller than a cell that are found within it
what is a tissue ?
group of similar cells that carry out the same function
what is a virus ?
an ultramicroscopic infectious non-cellular organism that can replicate inside the cells of living hosts, with negative consequences
when cells are ready to divide, what happens to DNA?
it copies itself, coils, and condenses to form the chromosomes you see in micrographs
how many chromosomes are in a human body cells ?
46 chromosomes, arranged into 23 pairs
what are the female sex chromosomes referred to ?
XX - as they’re identical in shape
what are the male sex chromosomes referred to ?
XY - as one of the chromosomes is a different shape
when do cells divide ?
- when an organism grows
- when an organism is damaged and needs to produce new cells
explain the cell cycle
cell growth
DNA synthesis - chromosomes are now double stranded
further growth occurs and DNA is checked for errors
mitosis
cytoplasm separates - 2 cells are formed
temporary cell resting period or cell stops dividing
explain mitosis
cell begins to divide
DNA replicates to form 2 copies of each chromosome
nuclear membrane breaks down, chromosomes line up across the centre of the cell
one set of chromosomes is is pulled to each end of cell and nucleus divides
cytoplasm and cell membranes divide to form 2 identical cells