Scaffolds & TE Constructs

Question 1

Scaffold wishlist

Answer 1

Nonantigenic, noncarcinogenic
nontoxic
biocompatible
immobilise transplanted cells
direct migration or prolif of cells via surface properties or soluble molecules

reproducibility and quality control
cost and speed
shelf life and stability

Question 2

Scaffold design considerations?

Answer 2

Porosity/pore size
Pore interconnectivity
Morphology/architecture (fibre spacing)

Question 3

Porogen leaching

Answer 3

Particles dispersed in polymeric and monomeric solution, gelling or fixing or structure, removal of template

Question 4

Phase separation

Answer 4

TIPS (process?)

PIPS (graph?)

Question 5

Electrospinning method and advantages

Answer 5

High voltage applied to fluid jet, fluid travels to region of lower potential
dimension similarities to ECM (high diff, low FBR)
high SA
increased diffusion
contact guidance (neurons)

Question 6

Fused deposition modelling

Answer 6

3D printer fuses build and support material

Question 7

Liquid-frozen deposition manufacturing

Answer 7

Low temp work chamber, nozel in xyz plane

Question 8

Stereolithography

Answer 8

laser beam, resin reservoir, platform in z axis

Question 9

Digital light processing

Answer 9

Upside-down stereolith

Question 10

Selective laser sintering

Answer 10

Stereolith with powder leveling roller

Question 11

Challenges with bioprinting

Answer 11

Decreased cell viability due to shear or extensional stress
Total build time
O2 supply during and after printing
Vascularisation