Bioreactors Flashcards
1
Q
Bioreactor definition
A
a device in which biological and/or biochemical processes develop under closely monitored and tightly controlled coniditions
2
Q
Functions?
A
Establish spatially uniform cell concentrations
control conditions in culture medium
provide physiologically relevant signals
facilitate mass transfer b/n cells & environment
3
Q
Key applications?
A
Cell seeding onto scaffolds
Maintaining adequate mass transport in seeded constructs
cell prolif and diff
laying down ECM
physical conditioning of developing tissues
4
Q
Challenges in design?
A
Oxygenation and CO2 removal
Provision and removal of macromolecules (cytokines and chemokines)
Overall physiological perfusion rates and uniformity of flow distribution down to functional subunit dimensions (100um)
