Cell Culture & Nutrition Flashcards
1
Q
How to establish a primary culture?
A
Enzyme digestion - cell culture
Finely chopped - primary explants
Further dissection - organ culture
2
Q
Cell culture parameters to consider
A
Finite vs continous normal or transformed species growth characteristics availability phenotypic expression stability
3
Q
Subculture protocol?
A
-
4
Q
Cell number graph
A
-
5
Q
Cryopreservation graph
A
-
6
Q
Cell culture medium components
A
water balanced salts energy suppliers buffers antifungal/bacterial substances AA supplements vitamins, cytokines, growth factors, hormones
7
Q
Oxygen delivery limitations in culture
A
Low solubility in media
Diffusion from gas phase to cell surface
