Saturation

Question 1

What is the Law of Diminishing Returns?

Answer 1

If we generate more mutants, we aren’t necessarily going to get more genes

Question 2

What is the divergence point?

Answer 2

The number of genes we can find is essentially maxed out and we aren’t wasting time generating more mutants

Question 3

How do we determine if we are at the divergence point?

Answer 3

Use the empirical formula: f(0) = e^-m

Question 4

Why is the empirical formula flawed?

Answer 4

It assumes all loci are equally mutable, which isn’t true

Question 5

What is f0 in the empirical formula?

Answer 5

How many genes involved in the process of interest haven’t been identified yet

Question 6

What is m in the empirical formula?

Answer 6

The mean number of alleles recovered for already identified genes

Question 7

How do we know if we are at saturation?

Answer 7

m is > or = 5

Question 8

What do we do if m is less than 5?

Answer 8

Calculate f0 and decide if we have live with that amount of genes missing