C elegans transformation

Question 1

How do you distinguish hermaphrodite worms from male worms?

Answer 1

Hermaphrodites have thin tails, males have fan shaped tails

Question 2

Do we transform male or hermaphrodite worms?

Answer 2

Hermaphrodites

Question 3

What type of transformation do we typically do for worms?

Answer 3

Binary

Question 4

What are the two vectors for worm transformation?

Answer 4

One contains the gene of interest and the other contains the selectable marker

Question 5

What are the two ways to use a selectable marker in a worm?

Answer 5

1. Can have a dominant mutation as the selectable marker and transform WT worms
2. Can have WT sequence and transform mutant worms

Question 6

Why do we have the sequence of interest and the selectable marker on separate vectors?

Answer 6

They will combine to form large arrays that we can integrate into the genome by exposing the transformants to ionizing radiation