Sample And Sample Handling (CRF) Flashcards
Samples are tested where?
In the clinical research facilities (CRF) in the hospital
Different levels of lab safety.
Standard PPE - low prevalence area
Full PPE- high prevalence area
Enhanced PPE- confirmed case eg COVID19
Types of clinical waste stream
Recycling bin
Clinical waste bin
Sharps bin
Summary of sampling
Sample collection
Sample processing
Aliquoting and transport
Storage
Thawing
Metabolómics analysis
Long-term storage at what temperature?
80°C
Short-term storage at what temperature?
- 20°C,4°C
Study design and protocol aim:
To give clear picture of what you need to do with the samples
Tells yo what the study is about and how to handle the samples e.g. Storage
If you deviate from the study/protocol you need to?
Inform the company and a reason for why
Different types of centrifuges
Class 1-3
Class 1 centrifuge
Outer air is drawn in over equipment or sample and aerosol containing air is sucked out through a HEPA filter which sterilises as it exits
Class 2 centrifuge
Has a fan blowing sterile air from one direction to another where it gets sucked up, filtered and sterilised. Protects both the specimen and the handler from contamination (most commonly used)
Class 3 centrifuge
Offers maximum protection for handler; leak-tight, double-filter system or filter and incineration, for highly infectious material - biosafety level 4 materials
Handling dry ice
Heavy duty gloves
Ice scoop
Ice bucket
Visor
Common sample problems
Fibrin clot
Hemolysis
Gel separation failure
Fibrin clot
Fibrin is an insoluble protein that is produced in response to bleeding and is the major component of the blood clot
