Safety and Microscope Flashcards

1
Q

Base of Microscope

A

gives stability to microscope

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2
Q

Ocular/eye piece lens of Microscope

A

viewing lenses to see microscope slide images

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3
Q

Neck/arm

A

holds eyepiece and nosepiece of microscope

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4
Q

Objectives

A

has scanner, low, and high power; varies the magnification of slides

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5
Q

Stage of Microscope

A

holds slide under objective lense, holds to be adjusted

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6
Q

Stage Clips of microscope

A

secures slides on the stage under the objective lenses

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7
Q

Diaphragm of Microscope

A

adjust the light given through the stage for the slide being viewed

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8
Q

Light Source of Microscope

A

provides adequate light for a clear image of a slide while increasing magnification

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9
Q

Coarse adjustment of Microscope

A

lowers and raises the stage, general focus

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10
Q

Fine adjustment of Microscope

A

lowers and raises stage of microscope, used on 10x and 40x magnification for detailed image

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11
Q

Nosepiece of Microscope

A

adjusts objectives to different levels of magnification

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12
Q

Working Distance

A

the distance between the bottom of the objective lens and the stage

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13
Q

Depth of Field

A

the distance in which the specimen appears to be in focus

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14
Q

Field of View

A

the area that can be seen through the ocular lens, circle of light

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15
Q

Resolution

A

the minimum distance in which two points can be separate and still be distinguished

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16
Q

Monocular

A

scope only has one ocular lens for viewing specimens

17
Q

Binocular

A

scope has two ocular lens for viewing specimens

18
Q

What do you expect in terms of the working distance of the microscope when you use each? In other words, which one do you think has the shortest working distance?

A

I imagine from the scanner to low power to high power the magnification of an image will increase and the working distance will decrease; the high scanner has the smallest working distance

19
Q

Objective lenses magnification

A

scanner: 4x
low power: 10x
high power: 40x

20
Q

What do you expect in terms of the working distance of the microscope when you use each? In other words, which one do you think has the shortest working distance?

A

I imagine from the scanner to low power to high power the magnification of an image will increase and the working distance will decrease; the high scanner has the smallest working distance

the field of view decreases as magnification increases

21
Q

How do compound scopes work?

A

Compound scopes rely on a series of mirrors to refract light through the specimen; This results in an inverted
image; It also affects the direction the
image moves in the eyepiece when you move the stage!

22
Q

What are general requirements/protocols in the lab?

A

legs completely covered from head to foot, closed shoe with firm bottom, safety glasses for designated labs, proper attire

23
Q

What is the magnification of our ocular/eyepiece in Biological Foundations lab?

A

10x(multipled with eyepiece lens)