Safety and Microscope Flashcards
Base of Microscope
gives stability to microscope
Ocular/eye piece lens of Microscope
viewing lenses to see microscope slide images
Neck/arm
holds eyepiece and nosepiece of microscope
Objectives
has scanner, low, and high power; varies the magnification of slides
Stage of Microscope
holds slide under objective lense, holds to be adjusted
Stage Clips of microscope
secures slides on the stage under the objective lenses
Diaphragm of Microscope
adjust the light given through the stage for the slide being viewed
Light Source of Microscope
provides adequate light for a clear image of a slide while increasing magnification
Coarse adjustment of Microscope
lowers and raises the stage, general focus
Fine adjustment of Microscope
lowers and raises stage of microscope, used on 10x and 40x magnification for detailed image
Nosepiece of Microscope
adjusts objectives to different levels of magnification
Working Distance
the distance between the bottom of the objective lens and the stage
Depth of Field
the distance in which the specimen appears to be in focus
Field of View
the area that can be seen through the ocular lens, circle of light
Resolution
the minimum distance in which two points can be separate and still be distinguished
Monocular
scope only has one ocular lens for viewing specimens
Binocular
scope has two ocular lens for viewing specimens
What do you expect in terms of the working distance of the microscope when you use each? In other words, which one do you think has the shortest working distance?
I imagine from the scanner to low power to high power the magnification of an image will increase and the working distance will decrease; the high scanner has the smallest working distance
Objective lenses magnification
scanner: 4x
low power: 10x
high power: 40x
What do you expect in terms of the working distance of the microscope when you use each? In other words, which one do you think has the shortest working distance?
I imagine from the scanner to low power to high power the magnification of an image will increase and the working distance will decrease; the high scanner has the smallest working distance
the field of view decreases as magnification increases
How do compound scopes work?
Compound scopes rely on a series of mirrors to refract light through the specimen; This results in an inverted
image; It also affects the direction the
image moves in the eyepiece when you move the stage!
What are general requirements/protocols in the lab?
legs completely covered from head to foot, closed shoe with firm bottom, safety glasses for designated labs, proper attire
What is the magnification of our ocular/eyepiece in Biological Foundations lab?
10x(multipled with eyepiece lens)