Safety

Question 1

What items are not allowed at the work bench?

Answer 1

• Food, drinks, gum, mints

* Purses, backpacks, jackets

Question 2

While working in the micro lab ___________ your mouth, eyes or other susceptible areas.

Answer 2

touch

Question 3

Describe the proper attire for working in the micro lab.

Answer 3

• closed toe shoes
• lab coat
• hair tied back
• gloves if necessary

Question 4

You cannot apply ___________ while working in the micro lab.

Answer 4

Cosmetics/lip balm/lotion

Question 5

When do you wash your hands?

Answer 5

At the beginning of lab, after handling living microbes, before leaving lab, and after reentering the lab.

Question 6

How and when is disinfectant used?

Answer 6

At the beginning and end of lab, it is applied and allowed to air dry.

Question 7

When leaving the lab make sure to ____________.

Answer 7

Push chairs under the bench

Question 8

When do you notify the instructor if something is wrong?

Answer 8

• accidents; spills, breakage,
• Injuries
• misconduct
• exposure to toxic chemicals, harmful microbes, or bodily fluids

Question 9

What do you do if you drop a culture and the container breaks on the ground?

Answer 9

immediately tell the instructor and cover the area with paper towels then saturate it with disinfectant

Question 10

Cultures are __________ removed from the lab.

Answer 10

never

Question 11

How do you properly label a culture?

Answer 11

• name/initals
• instructor name/initals
• date
• organism id if known

Question 12

How do you dispose of a plastic petri dish?

Answer 12

place it in the red biohazard bin

Question 13

What do you do with glass test tubes once you are finished using them?

Answer 13

The writing must be removed with alcohol, and the test tubes must be stored upright in the wire basket in the metal tub located on the contamination cart, DO NOT REMOVE CAPS OR EMPTY CONTENTS.

Question 14

How do you dispose of pipettes?

Answer 14

pipettes are placed in the metal pitcher labeled “pipettes” on the contaminant cart. PIPETTE TIP MUST BE POINTING DOWNWARD.

Question 15

How do you dispose of wet mount slides?

Answer 15

Wet mounts contain live microbes, the cover slip must be removed, and placed in the small metal can that says “coverslips”. Then deposit the slide in the beaker that says “10% Bleach” on it.

Question 16

What do you do with heat fixed slides once you are finished with them?

Answer 16

Hand wash the slides with water and comet cleaner, dry and place back in slide box.

Question 17

Where are paper towels disposed of?

Answer 17

in regular trash cans UNLESS CONTAMINATED THEN THEY SHOULD BE PLACED IN THE RED BIOHAZARD BIN.

Question 18

Gloves are placed where when you are finished using them?

Answer 18

in the red biohazard bin, make sure to wash hands after removing gloves.

Question 19

Where does broken glass go?

Answer 19

UNCONTAMINATED glass goes in broken glass box, CONTAMINATED glass will be disposed of according to instructor’s directions.

Question 20

How should on handle a bunsen burner?

Answer 20

• Never touch the top of the burner
• shut gas all the way off when done
• watch out for cabinets, clothing, hair, certain reagents, stains and alcohol.

Question 21

Common drawer

Answer 21

Contains equipment for all students to use; striker, ruler, grease pencil, lens paper, bibulous paper

Question 22

Student drawer

Answer 22

Contains slide box, coverslip box, loop, needle, forceps, eye dropper

Question 23

Test tubes must be _____________ at all times.

Answer 23

• carried in test tube racks

* Label the tube with the type of media asap

Question 24

When grabbing new media check to see if the media is ___________ then ________ of it properly if so.

Answer 24

• Contaminated

* dispose

Question 25

What is the slides are not deposited in 10% bleach?

Answer 25

• slides with india ink on them; have their own separate container.
• heat fixed smears which need to be hand washed