Aseptic Technique and Bacterial transfer

Question 1

Sepsis

Answer 1

bacterial growth

Question 2

Aseptic technique must always be followed to prevent _______________.

Answer 2

contamination of pure cultures

Question 3

Pure culture

Answer 3

contains only a single species of organism

Question 4

Aseptic technique

Answer 4

the methods to obtain, maintain and transfer bacterial cultures

Question 5

Media and containers are ________ during _____________.

Answer 5

• sterilized

* media preparation

Question 6

Work as _________ and __________ as possible. Avoid _________or other distractions.

Answer 6

• quickly
• safetly
• talking

Question 7

All media and containers must be kept covered why?

Answer 7

to prevent entry of microbes

Question 8

What must you do with transfer tool before collecting bacteria and after collecting bacteria before the tool leaves your hand?

Answer 8

the tools must be sterilized

Question 9

How do you avoid making aerosols?

Answer 9

letting transfer tools cool before collecting bacteria

Question 10

_________ and ____________ are never placed on the work surface.

Answer 10

• petri plate lids

* test tube caps

Question 11

Always flame the ______ of the _________ before you insert and after you withdraw the transfer tool.

Answer 11

mouth of the tube

Question 12

Never hold __________ by the caps. And never _______ them, just tap them with your finger.

Answer 12

• tubes

* shake

Question 13

Broth culture

Answer 13

bacteria suspended in solutions, usually containing nutrients to support the growth of microbes. They are contained in test tubes with caps

Question 14

Before using media check it for contamination by

Answer 14

• looking for odd colors or growth on plates or turbidity in broth tubes

Question 15

Why is the mouth of the tube swept over the flame?

Answer 15

to prevent air caring microbes from entering the tube, convection; hot air rises

Question 16

Tubes are held _________ when transferring bacteria.

Answer 16

slightly tilted

Question 17

Slants

Answer 17

testubes containing NA. Hot NA is poured in plates and allowed to cool at an angle

Question 18

Slants have ________ like broths and___________________ must be passed through the flame.

Answer 18

• lids

* mouth of the tube

Question 19

Which is easier to detect contamination, slants or broths?

Answer 19

slants

Question 20

What are slants best for?

Answer 20

they are favorable for longer storage of microbes , because they do not dry out as fast as plates.

Question 21

Why is the slant at an angle?

Answer 21

to allow for a larger surface area for culturing cells

Question 22

Which is easier to detect contamination, Plates or slants?

Answer 22

plates

Question 23

What is good about petri plates?

Answer 23

provides a large surface area and is good for separating microbes into single colonies.

Question 24

Petri plate lids are __________removed from plate and never set on the work bench.

Answer 24

*never fully

Question 25

Steps for aseptic transfer plate to broth.

Answer 25

1. Light bunsen burner
2. flame loop
3. let cool for ~20 sec
4. Open petriplate lid and hold it at an angle over the plate
5. rub a colony
6. grab the sterile tube and open the lid keep lid in hand
7. hold tube at angle and flame mouth
8. dip loop with bacteria into broth without touching sides of the tube
9. Flame mouth of tube and replace lid.
10. flame loop