RP8 - named factor on chloroplasts Flashcards
1
Q
Why should the leaf midrib or leaf stalk in the blender
A
No chloroplasts present
2
Q
What was the purpose of using a blender?
A
break open cells
3
Q
Why is it important that the isolation medium was cold?
A
- Slows enzymes activity
- Enzymes released during blending
- Chloroplasts not damaged
4
Q
Why is it important that isolation medium is isotonic?
A
- Same water potential
- Chloroplasts don’t burst
5
Q
Why should you wrap tube in aluminium foil?
A
Prevent entry of light
6
Q
A