RP1 - Enzymes Flashcards
1
Q
Explain why buffer solutions are used?
A
Maintain constant pH
2
Q
You left test tubes in water bath for 10 minutes before enzyme was added to milk powder solution explain why?
A
To reach the temperature in which the reaction will take place
3
Q
Describe and explain what you did to make temperature of water baths as reliable as possible (2)
A
- Measure temperature and and hot and cold water when appropriate
- Try to keep temperature close to required
4
Q
Explain why you set up three experiments at each temperature? (2)
A
- Calculate a more reliable mean
- So anomalous data can be found
5
Q
What would a control without the enzyme show?
A
To show the digestion was due to the enzyme not the temperature
6
Q
Suggest a more appropriate control than no enzyme?
A
Boiled enzyme
7
Q
Suggest one better way of deterring when solution of milk powder goes clear?
A
Use a colorimeter to measure absorption
