RP2 - Microscopes Flashcards
1
Q
Describe how the scientist could have used the temporary mounts of leaves to detemine the mean number of chloroplasts in mesophyll cells of a leaf. (3)
A
- Count all cells in view
- Count the number of chloroplasts
- Number of chloroplasts / number of cells
2
Q
Why is it important sections are thin? (2)
A
- Allows light to pass through
- Stains can work more easily
3
Q
Why do you make sure coverslip doesn’t move sideways
A
Specimen doesn’t become damaged
4
Q
Describe how temporary mounts are made?
A
- Create a thin layer of sample
- Stain the specimen
- Push down on blot using coverslip
5
Q
What is purpose of heating acid?
A
Break up tissues in cells
6
Q
How to make sure mitotic index is accurate?
A
- Look at large numbers of field of view
- So its representative
