Role of Microbiology Lab Flashcards
what are the two functions of the microbiology lab
clinical
epidemiological
what is the clinical aspect of the microbiology lab
diagnosis of infection in an individual patient for everyday management
what is the epidemiological aspect of microbiology labs
support for infection prevention and control in searching for source and route of transmission of HAI (hospital acquired infections)
what are the minimal requirements for microbiology services
Set up inside the facility
Available everyday, including sundays and holidays
able to examine blood, CSF, urine, stool, wound exudate and perform basic serological tests
identify most common bacteria and fungi to species level
perform susceptibility testing
perform basic phenotyping
what are classical biological methods of diagnosing infection
direct smear
culture
antigen detection
what are ways in which infection can be diagnosed in the lab
sensitivity testing
antibody detection
molecular methods
classical bacteriological methods
what is the process for bacterial identification
specimen
direct examination
culture
pure culture and identification to species
species name established
typing or fingerprinting for taxonomic or epidemiological purposes
what are the two types of shapes of bactieria
rods
cocci
what is the process of gram staining
prepare bacteria on glass slide
stain with crystal violet and rinse
treat with grams iodoine
briefly decolourise with acetone or ethanol
counter stain with safarin
blot dry and view under oil immersion
what colour will gram positive cells be
violet
what colour will gram negative cells be
pink
what are HAIs
hospital associated infections
what are the 4 roles of microbiology labs in prevention and control of HAIs
outbreak investigation
surveillance of HAIs
alert microorganism reports
design antibiotic policies
what is included in HAIs surveillance
produce routine reports of bacterial isolates
a baseline incident can be determined
what are some alert microorganisms
MRSA
c dificile
COVID 19
