Role of hydrogen acceptors. Flashcards

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1
Q

What is the process called in which hydrogen atoms are removed from molecules?

A

Dehydrogenase enzymes catalyse the reactions in reactions called dehydrogenation.

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2
Q

How do redox indicators work?

A

The are a different colour when they are reduced as to when they are oxidised. They collect the hydrogen instead of the NAD and FAD and so show that respiration is occurring.

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3
Q

State 3 redox indicators.

A

Methylene blue
Triphenyl Tetrazolium Chloride (TTC)
DCPIP

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4
Q

Give the colour change for each.

A

Methylene blue - Blue to Colourless
TTC - Colourless to Pink
DCPIP - Blue to Colourless

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5
Q

Give practical details on how to carry out this experiment.

A

1) Crush soaked peas to a paste in a pestle and mortar and place in a boiling tube.
2) Repeat with boiled peas
3) Make up some methylene blue of a suitable concentration
4) Add 2cm³ of methylene blue to each boiling tube and place in a water bath at 35ºC.
5) Observe the colour change over time.

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6
Q

What are various experiments Redox indicators can be used for?

A

1) The example above
2) The effect of dehydrogenase activity on adding an intermediate of the krebs cycle e.g. succinate
3) Effect of malonate - competitive inhibitor of succinate dehydrogenase

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