Rodgers 2-19

Question 1

How are SMC proteins released from the chromosomes

Answer 1

kleisin proteins are cleaved to open up the hinge

Question 2

Protein that excludes the condensin from the nucleus until S phase

Answer 2

Ku protein

Question 3

What interaction helps condensin loop the dna together?

Answer 3

multimerization with each other

Question 4

What are the 3 major features of the nuclear envelope?

Answer 4

Continuous with the ER, Double bilayer, nuclear pores

Question 5

Does the nucleus remain closed during mitosis of higher eukaryotes?

Answer 5

No - higher eukaryotes undergo “open mitosis,” unlike lower eukaryotes (ex: fungi)

Question 6

How does the nuclear envelope dissolve during mitosis

Answer 6

The nuclear envelope “blebs off” into the ER and the nuclear proteins disperse (ex: laminins, nuclear pore proteins)

Question 7

Which proteins are recruited to the chromosomes during mitosis

Answer 7

INM proteins and nucleopore proteins

Question 8

What protein is responsible for recruiting the nuclear envelope back into place?

Answer 8

nuclear pore proteins

Question 9

Units of the centrisome

Answer 9

centrioles

Question 10

Another name for the centrisome

Answer 10

MTOC (mitotic tubule organizing center)

Question 11

Which pole of microtubules is the growing/polymerizing end

Answer 11

[+]

Question 12

What is the protein which attaches the sister chromatids to the mitotic spindle

Answer 12

kinetochore

Question 13

What is the primary mechanism of microtubule growth during mitosis

Answer 13

“search and capture” from the centrosome origin

Question 14

To what DNA segments are the kinetochores affiliated?

Answer 14

kinetochores form on the alpha-satellite sequences repeated at the centromere

Question 15

How do heterochromatin differ in the centromere from the rest of the DNA

Answer 15

Specialized histone subunits [like the special H3 protein CENP-A] and other specialized proteins that form a layer atop the histone

Question 16

CENP-A

Answer 16

Histone protein essential for centromere formation

Question 17

What is the separating structure that forms between the segregating telophase nuclei

Answer 17

the MIDBODY RING; it forms by G protein activation by the mitotic spindles at the center

Question 18

ESCRT-III

Answer 18

The protein responsible for scission of the daughter cells. It polymerizes into a coil that pinches off the new membrane

Question 19

What is the CPC? How does it form?

Answer 19

Chromosome passenger complex forms by the interactions of opposite poles of mitotic tubules overlapping

Question 20

Proteins that make up the mid-bpdy complex

Answer 20

Actin, myosin, septin, anillin

Question 21

Abscission

Answer 21

separation of the daughter cells by ESCRT proteins