Rodgers 2-17 Flashcards
Describe the relationship between MinC,D, and E
ATP-bound MinC and D polymerize to a hlical structure at one end of the cell until MinE concentration (PHOSPHOTASE) accumulates and depolymerizes the structure. This process restarts at the other end of the cell in oscillation until separation/replication
What is the Z-ine made of
FtsZ
What protein does the bulk of the ‘pinching off’
FtsK- does the last bit of chr segregation
What are the features of FtsK
ATPase, motor protein, transmembrane protein,
When does E. coli start segregating new daughter chromosomes to the poles
Early in replication of genome
How do prokaryotes organize segregation of new chrs if there’s no mitotic spindle
MigS, MatS, MatP : various proteins anchor the terminus in the middle and the OriC’s to their respective quarterpoints
What proteins make up the histone octamer?
2 x H2A, H2B, H3, H4
What is the purpose of H1
Forms higher order structures from nucleosomes, bonds with N-terminals of histones
What features are prominent in histone proteins
Highly basic, positively charged (helps bind DNA), intrinsically disordered N-tails
How does modifying histones affect their interactions with the DNA
most modifications (methylated, acetylated, phosphorylated- opens DNA) change the histones’ charges
For what TWO REASONS does it matter to save the modifications on histones during replication?
- Maintains the cell’s identity 2. Structurally important to the replicating chromosome
How are histones’ integretity maintained during replication?
histone chaperones transfer one or two of the old histone proteins into new nucleosomes. The information carried by the old histones is sufficient to re-assign needed modifications
What are SMC proteins?
Structural Maintanence Proteins- of which class the condensin complex (stabilize chromosomes into loops) and cohesin (condense sister chromatids) belong
How is cohesin released from binding sister chromatids
separase is released from securin inhibitor to cleave Cohesin
