RNAseq

Question 1

Sequencing depth

Answer 1

Number of genes or molecules detected per cell

Question 2

Housekeeping genes

Answer 2

In molecular biology, housekeeping genes are typically constitutive genes that are required for the maintenance of basic cellular function, and are expressed in all cells of an organism under normal and patho-physiological conditions

Question 3

Library size

Answer 3

We define the library size as the total sum of counts across all genes for each cell, the expected value of which is assumed to scale with any cell-specific biases.