RNA + PROTEIN SYNTHESIS Flashcards
Where do we find RNA
in the nucleus and cytoplasm
As w DNA forms part of the ribosomes
What is the structure ofRNA
a single strand - polymer with nucleotides as monomers
What are the 4 different nitrogenous based in RNA
Adenine
Guanine
Cytosine
Uracil(replaces thymine)
What are the 3 types of RNA
-mRNA (messenger RNA) carries the genetic code from DNA in the nucleus to the ribosomes in the cytoplasm
-tRNA (transfer RNA) looks like a hairpin + has three exposed bases called the anticodon. It picks up the AA in cytoplasm and takes to ribosomes where protein synthesis occurs
-rRNA (ribosomal RNA) single strand and plays a role in control of protein synthesis
Name 3 differences between DNA and RNA (table form)
Double helix. | Single strand
Sugar is deoxyribose |. Sugar is ribose
ATCG. |. AUCG
What are the 2 main steps of protein synthesis
- transcription of DNA
- translation of RNA or proteins
What is protein synthesis
Process whereby proteins are manufactured in living cells
What is transcription
DNA never leaves the nucleus and protein synthesis occurs at ribosomes but a code for the synthesis must be transferred from DNA to mRNA which is able to leave the nucleus - the process of mRNA obtaining the code for protein synthesis from DNA is transcription
What is the process of transcription
- strands of DNA unzip
- enzyme RNA polymerase controls the transfer of the code from DNA to RNA and causes the weak hydrogen bonds to break
- free floating RNA nucleotides in the nucleoplasm bind with the free bases to form complementary mRNA
- each group of 3 nitrogenous bases on mRNA is a codon which codes for a particular AA
- the base triplet of mRNA will be complement to DNA (GGU was CCA)
- as the mRNA forms it moves away from DNA strand
- mRNA strand leaves the nucleus via pored in the nuclear membrane and taken to ribosomes
What is the process of translation
- the mRNA strand that was formed attaches to the ribosomes
- mRNA now provides a code for the linking of AA to form a specific protein.
- tRNA occurs in the cytoplasm and it picks up AA (bc of its anti codon (3 exposed bases) in cytoplasm and brings them to ribosomes
- the anticodon determines which AA will bind to tRNA
- a specific tRNA brings the AA for which it is coded, to the mRNA template (these are complementary)
-AA join in a chain - polypeptide - once chain is formed the tRNA breaks the bind with AA and moves away from the ribosome
- the tRNA is now ready to pick up another AA of the same type
- this process proceeds until 50 or more AA join and a protein is formed