RNA poly Ecoli Flashcards
RNA polymerase covers roughly how many base pairs
35
Movement of RNA Poly during transcription causes what
Positive supercoiling ahead of transcription and negative behind it
In a cell, how is supercoiling controlled
Topoisomerase
Name the 6 subunits of RNA polymerase
2 alphas
One beta and anti beta
One omega
One sigma
RNA pol lacks what so there is a high error rate
3’ to 5’ exonuclease
RNA binds to what regions to initiation transcription
Promoter regions
The 2 alphas are for
Assembly and binding to upstream elements
The beta is for
Main catalytic subunit
The b’ is for
DNA binding
The sigma is for
Directing enzyme to the promoter
The omega subunit is for
Protecting the polymerase from denaturation
The Tata sequences lie where for what subunit to bind
At -10 and -34 for sigma subunit
What rich sequences binds the alpha sub unit
A-T rich sequence upstream promoter element between -40 and -60 binds the alpha sub unit
A-T rich sequences promote what
Strand separation
The promoter sequences govern what and so effects what
Governs efficacy of RNA pol binding
Affects gene expression level
