Intro Flashcards
1
Q
UV absorption of nucelobases is due to what
A
Pi-pi* electronic transition
250-270nm
2
Q
Covalent bonds in DNA are formed via what
A
Phosphodiester linkages
3
Q
The backbone of DNA has what charge
A
Negative
4
Q
Backbone of RNA is stable or unstable
A
Unstable
5
Q
RNA lasts longer in water or cells
A
Water
6
Q
Linear polymers have no what
A
Branching or cross links
7
Q
The 5’ and 3’ ends of DNA have what groups
A
3’ end is -OH
5’ end is -PO4
8
Q
Exonuclease are what
A
Enzymes that hydrolyse only those phosphodiester bonds that are in the terminal positions of the nucleic acid
9
Q
Nuclei are
A
Nitrogenous base
10
Q
Processivity
A
Number of nucleotides added before dissociation
