RNA overview Flashcards
What are the differences between RNA and DNA sugars?
RNA sugars have two OH’s
How does the extra OH group on RNA sugars effect the nucleotides?
make them less stable
Whats the difference between RNA and DNA bases?
RNA uses U instead of T (u is less stable)
RNA is synthesizes in which direction?
5’ to 3’
What performs transcription? How?
rna polymerase does, reads template dna in 3” to 5” direction and add nuceltides in 5” to 3” directions
RNA polymerase unwinds how many bp of dna at a time?
17
About how much of the human genome is protein coding?
1-2%
What does UTR stand for?
untranslated region
What does RBS stand for?
ribosome binding site
What are regulatory sequences in DNA? Do they get transcribed?
are regions that determine where and when the protein coding region will be transcribed (like in what tissue and what time), no they don’t get transcribed
Does only the protein coding region get transcribed?
No, UTRs and introns, and RBS and terminators get transcribed
Do prokaryotes have introns?
no
What is an exon?
is included in mature RNA and translated to proteins
What happens to introns during translation?
get spliced out
What is the splicing machinery?
the spliceosome