RNA

Question 1

Regulation of Lac Operon

Answer 1

TF (required for start) only there when glucose is low. Repressor only leaves when lactose present. So really only expressed when glucose low and lactose present, so will start breaking down lactose

Question 2

AA that Might be Coded for More than is Present in Protein

Answer 2

Met (because can be cleaved off after coded for by AUG start codon)

Question 3

3 Euk RNA Pols (which kind of RNA they code for and where)

Answer 3

RNA Pol I - rRNA in nucleolus
RNA Pol II - mRNA in nucleoplasm
RNA Pol III - tRNA in nucleoplasm

Question 4

3 Inhibitors of RNA Pols (2 drugs and 1 toxin)

Answer 4

alpha-amanitin from Amanita phalloides inhibits RNA Pol II (euks). Causes hepatotoxicity
Rifampin inhibits prok RNA Pol (4 Rs - Rifampin, rRNA Pol, revs up cytochrome p450, red secretions)
Actinomycin D inhibits RNA Pol in euks and proks

Question 5

3 Processings before hnRNA is mRNA and can be exported from nucleus

Answer 5

Capping of 5’ end with 7methylguanosine cap
Polyadenylation of 3’ end (AAUAA with 200 As)
Splicing out of introns (remain in nucleus. Exons expressed)