Lab Techniques

Question 1

3 Steps of PCR

Answer 1

Denaturation
Annealing - DNA primer
Elongation - DNA Pol

Question 2

3 Steps of PCR

Answer 2

Denaturation
Annealing - DNA primer
Elongation - DNA Pol

Question 3

Gel Electrophoresis Direction/Size

Answer 3

Use PCR DNA products. Smaller strands go further towards + electrode, longer strands travel shorter. Proteins can also be tested, but not all have negative charge so it separates based on size and charge

Question 4

Southern Blot

Answer 4

DNA probe for DNA sample

Question 5

Northern Blot

Answer 5

DNA probe for RNA (mRNA) sample

Question 6

Western Blot

Answer 6

Antibody probe for protein sample

Question 7

Southwestern Blot

Answer 7

Oligonucleotide probe for DNA binding proteins (like TFs)

Question 8

Direct vs. Indirect ELISA

Answer 8

Direct: Use test Ab to see if a specific Ag is present in serum
Indirect: Uses either test Ag or Ab to see if a specific Ab or Ag, respectively, is present