RNA

Question 1

Where is mitochondrial DNA found?

Answer 1

Near mitochondrial membrane and is double stranded and circular.

Question 2

What did Garrod (1909) suggest?

Answer 2

Linked symptoms of an inherited disease with a persons inability to make a particular protein (Enzyme). The first to recognise Mendel’s laws in humans.

Question 3

What do genes dictate

Answer 3

Phenotypes

Question 4

What did Beadle and Tatum study?

Answer 4

Mutations in the pathway in which arginine is synthesised.

Question 5

What is transcription catalysed by?

Answer 5

An enzyme - RNA polymerase

Question 5

Growing conditions for mutants?

Answer 5

Supplied with a compound made after defective step

Question 6

What does RNA polymerase do?

Answer 6

Unwinds the double helix and makes a complementary copy of one strand (template strand) of DNA

Question 7

What does RNA polymerase use?

Answer 7

Triphosphates to supply the nucleotide units to incorporate the growing RNA chain

Question 8

in what direction does DNA and RNA replication grow in?

Answer 8

5’ to 3’ only

Question 9

Site of initiation?

Answer 9

Promoter containing a particular sequence of bases.

Question 10

Initation in prokaryotes

Answer 10

RNA polymerase itself recognises and binds to the promoter site

Question 11

Initiation in eukaryotes

Answer 11

Set of proteins (transcription factors) are involved in the binding of RNA polymerase to the promoter

Question 12

How does elongation proceed?

Answer 12

By local unwinding of 1-2 turns of DNA and addition of nucleotide units.

Question 13

what does mRNA splicing remove after capping and tailing?

Answer 13

Non-coding regions (introns) so as to leave the coding regions (Exons)