Photosynthesis Flashcards

1
Q

What is photosynthesis?

A

A process than converts Co2 and H2o to carbohydrates

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2
Q

What are phototrophs?

A

Photosynthetic organisms

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3
Q

Where is solar energy captured?

A

Int eh chemical form of ATP and NADPH

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4
Q

What are ATP and NADPH used to convert?

A

CO2 to hexose phosphates

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5
Q

Light dependent reactions…

A
  • Are membrane bound
  • Release O2 from splitting 2H2o molecules, with H+ from H2O used in the Chemiosmotic synthesis of ATP, and Hydride ion (H:-) from H2O reduces NADP+ to NADPH
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6
Q

Dark reactions…

A

Occur in solution, reduce gaseous CO2 to carbohydrate and require energy of NADPH and ATP

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7
Q

Chloroplast structure

A

Two surrounding membranes, internal membrane stacking

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8
Q

Chlorophylls are the…

A

Most abundant and important in light harvesting

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9
Q

What does chlorophyll contain?

A

A tetrapyrrole ring (chlorin) similar to heme but contains Mg2+

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10
Q

What do antenna pigments extend?

A

The range of light capture, especially in the green where chlorophyll absorbs poorly

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11
Q

What do antenna pigments and chlorophylls exchange?

A

Light energy (excited electrons) until captured

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12
Q

What are the two photosystems?

A

Photosystems I (PSI) and Photosystems II (PSII)

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12
Q

PSI and PSII

A
  • Contain many proteins and pigments embedded in the thylakoid membrane
  • Operate in series, connected by cytochrome bf complex
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13
Q

Electrons are conducted from?

A

H2o to NADP+

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14
Q

What do PSI and PSII both contain?

A

A reaction centre that is the site of the photochemical reaction. Chlorophylls in each relation centre are paired to capture light

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15
Q

What is PSI paired to capture?

A

P700, absorb light maximally at 800nm

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16
Q

What is PSII paired to capture?

A

P680, absorb light maximally at 680nm

17
Q

What is the Z-scheme?

A

Path of electron flow and reduction potentials of the components in photosynthesis

18
Q

What does the absorption of light energy convert?

A

P680 and P700 (poor reducing agents) to excited molecules (good reducing agents)

19
Q

Mitchells chemiosmosis theory

A

Conversion of energy from electron transport via formation of a transmembrane electrochemical gradient

20
Q

Photophosphorylation

A

Synthesis of ATP which is dependant upon light energy

21
Q

Chloroplast ATP synthase consists of?

A

Two major particles, CF0 and CF1

22
Q

Cyclic electron transport

A

Yields ATP but not NADPH, thus balancing the need for 3 ATP for every 2 NADPH

23
Q

What does cyclic flow increase?

A

The protonmotive force and increases ATP production, but no NADP+ is produced

24
How were dark reactions identified?
Fast Co2 injection Rapid 'quench' (perchloric acid) to stop reactions
25
What does 2D chromatography improve?
Separation of intermediates
26
What is the key enzyme in Calvin-Benson cycle?
RuBisCo
27
What does RuBisCo use?
O2 to catalyse a competing oxygenation reaction
28
What does photorespiration recyle?
The toxic products of the oxygenation reaction; it consumes NADH, ATP to give glyoxylate, serine, glycine and CO2
29
What are some alternative photosynthetic strategies?
Cyanobacteria form carboxysomes, some algae form pyrenoids some angiosperms have evolved C4 and CAM photosynthesis
30
What are carboxysomes?
Semi-crystalline protein structures incorporating RuBisCO
31
What are Pyrenoids
Membrane-bound structures found in the chloroplasts of some algae. Like carboxysomes, include RuBisCo and concentrate CO2
32
what does C4 photosynthesis fix?
HCO3- with pyruvate into malate.
33
Where does c4 photosynthesis transport malate?
To a second cell type for decarboxylation and refixation by RuBisCo
34
Why is C4 photosynthesis more efficient?
Because it fixes HCO3- first and avoids O2
35
What does C4 photosynthesis depend on?
Spatial separation of the C4 and C3 (RuBisCo) fixation reactions. The only major C4 crop is maize
36
What does CAM photosynthesis depend on?
Temporal separation of the C4 and C3 fixation reactions
37
What does CAM require?
Fine control of gene expression over the day-night cycle
38
What cycle does CAM use?
An inverted cycle of stomatal opening/closing to save water and concentrate CO2 in the leaf
39