Riyaz - BIO - Flashcards

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1
Q

Which of the following Vitamins are water soluble?

A

Vitamin ADEK - Fat Soluble & Vitamin B,C water soluble

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2
Q

What would the results be of another experiment Z was conducted based on the results from experiment X and Y?

A

If there is a experimental question where they ask you to predict a value of a experiment, see the flow of the experiment and relate it to the flow of the previous experiments conducted in the passage and reason within the text

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3
Q

What is the function of Reverse Transcriptase and how does it help a virus stay hidden from patients undergoing Nucleotide Analogs?

A

Reverse Transcriptase - An enzyme that catalyzes the formation of DNA from an RNA template in Reverse Transcriptase.

Itmutates to prevent incorporation of analog drugs decreasing the effects of drugs given which is why AIDS patients are still not curable

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4
Q

How does a Antibody Prevent a virus from entering the cell?

A

Antibody production prevent Virus from entering the cell by:

1) Injecting a small amount of “live” Virus
2) Injecting “heat Killed” Virus
3) Injection of part of the outer coat of the virus.

Antibody production against revere transcriptase would not prevent the virus from entering the cell

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5
Q

How do you tell the indications of an experiments results?

A

Try to assess what is going on in the experiment and create a outcome of what was experimented with what and what was the result?

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6
Q

If Bacteria X was placed in a medium and then centrifuged out, and then bacteria Y was placed in after, and the growth of Bacteria Y had a different outcome from the control what was the mode of communication of the bacterias?

A

If the Passage says that the medium of one bacterial culture has affected the second bacterial growth after the first bacteria was removed, understand there most be a secondary mechanism of communication of the bacteria’s communicating such as a protein secreted

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7
Q

How to tell the if one concept relates to another on the test day?

A

Know the relationships between various mechanisms in the body exist that may relate to a similar concept.

Ex. hormones and receptors are similar to Nerve cells as they elicit similar responses too as well as two communicating bacterias

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8
Q

What happens to a bacteria if it is deficient in transcription?

A

Replication, transcription and translation are important for any bacterial or eukaryotic cell to replicate and survive!

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9
Q

if a bacteria doubles every x minutes how much will there be present in y hours with an initial of 500.

A

Be sure to make sure how to use logarithemic calculations for test day for bacterial growth if doubles every x amount of minutes

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10
Q

How do lysozymes work and what do they break down in the cell?

A

Understand that Lysozymes break down polysaccharides, proteins, nucleic acids and lipids and usually fuse with the endocytotic vesciles and digest the substances then. Sometimes they may just digest the cell itself, but if something is breaking down a substance inside the lysozyme it must be present in the lysozyme itself

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11
Q

If enzyme A binds substance X, and inhibits its growth, would it do the same for Enzyme C?

A

Enzymes are specific for their substrates and will only bind to that substrate not any other substrate!

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12
Q

What is the flagella? And if a cell does not contain a nucleus what organelle may it contain?

A

If a cell does not contain a nucleus it is similar to a bacterial cell and most likely not have any other membrane bound organelles but will contain the Flagella!

Flagella is a organelle

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13
Q

In Eukaryotic cells, where may DNA replication take place?

A

DNA replication may occur in the nucleus as well as the Mitochondria! Mitochondria have their own type of DNA replication similar to that of bacteria (endosymbiotic theory).

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14
Q

When the Sodium Potassium pump is active, which ion has a higher concentration inside the cell?

A

If no other pump is active on a cell except the Sodium Potassium pump and the Sodium Glucose pump, Then the Potassium concentration will always be higher inside the cell.

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15
Q

Know that when a inhibitor of a enzyme or protein is present in data the presence of it usually will be denoted by a + and absence by a -

A

Know that when a inhibitor of a enzyme or protein is present in data the presence of it usually will be denoted by a + and absence by a -

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16
Q

Read axis of graphs and captions of figures to understand what is being displayed in which perspective. Read in the passage the sentences which refer to the displayed figures.

A

Read axis of graphs and captions of figures to understand what is being displayed in which perspective. Read in the passage the sentences which refer to the displayed figures.

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17
Q

What does retrograde transport mean?

A

Read each key word importantly and understand retrograde transport is of which means things occur in the reverse direction.

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18
Q

In regards to the adaptive immune system, can you transfuse mouse antibodies into humans?

A

A human body will reject foreign substances because it will not recognize them and build antibodies against them. This is due to the adaptive immune system and repetetive usage will cause a cascade of antibodies and possibly hypersensitivity.

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19
Q

Neutrophils must adhere to endothelium before traveling through, read the answer choices properly and try to reason back with information given in the passage. Remember key words.

A

Neutrophils must adhere to endothelium before traveling through, read the answer choices properly and try to reason back with information given in the passage. Remember key words.

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20
Q

What part of the DNA process does splicing occur?

A

Splicing of introns and removal of remaining pieces of exons occur from DNA –>mRNA and is a step production of mRNA from transcripted pre-mRNA.

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21
Q

What is the Le’ Chatelier’s principle?

A

Le’ Chateliers principle states, the equilibrium of a reaction will favor products when the conc. of products is kept low or the forward direction. Or in general when stress is added to a system, the system will try to compensate and bring the reaction to equilibrium.

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22
Q

What content a PCR primer mostly entail?

A

PCR primers have a high GC content and G and C base pairs at 5’ and 3’ end.

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23
Q

What is Nondisjunction? Where does it occur?

A

Nondisjunction is the failure of homologous chromosomes to seperate during Anaphase 1 of meiosis or the failure the failure of sister chromosomes to seperate during Anaphase 2 of Meiosis.

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24
Q

Which amino acids have 2 nitrogens in their formula? Which amino acids are zwiterionic at pH 7?

A

Only Glutamine (GLN), Asparagine (Asn), or Tryptophan (trp) have 2 nitrogen atoms in their formula and are zwiterionic at pH7.

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25
Q

Read the question stem properly and understand what it is asking. If there are few of something then there most likely may be a few response to that reaction.

A

Read the question stem properly and understand what it is asking. If there are few of something then there most likely may be a few response to that reaction.

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26
Q

Read data properly and understand the significant differences in the data given. Look out for NOT and EXCEPT questions - meaning every answer choice except the correct one is in line with the passage except for what the question is asking.

A

Read data properly and understand the significant differences in the data given. Look out for NOT and EXCEPT questions - meaning every answer choice except the correct one is in line with the passage except for what the question is asking.

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27
Q

Are tissue cells of single celled organisms capable of independent life?

A

Tissue cells 1)divide by Mitosis and are capable of 2)subcellular organelles, 3) metabolize nutrient molecules but are not capable of independent life.

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28
Q

what does a fatty acid composed of?

A

Fatty acid is composed of a hydrocarbon tail and a carboxyl head/

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29
Q

What happens if you inhibit Carbonic Anhydrase?

A

If Carbonic Anhydrase is inhibited in the body, there will be a build up of CO2 in the tissues.

30
Q

Which structure contains the alpha helices and beta sheets?

A

The alpha helices and beta sheets are in the secondary structure of a protein. Disruption may occur by a change in the amino acid in that protein. Proline also disrupts secondary structure.

31
Q

What happens if you change a hydrophobic residue to a more hydrophilic amino acid on the surface of a protein?

A

Changing a hydrophobic residue to hydrophilic residue on the surface of a protein will decrease entropic penalty and issues in protein folding

32
Q

Read the data carefully when asked about a experiment. The answer lies with in the data

A

Read the data carefully when asked about a experiment. The answer lies with in the data

33
Q

Difference between germline and somatic mutations?

A

When a mutation described in the passage is considered to be in only specific cancerous cells, it is hinting it is a somatic mutation not a germline and cannot be inherited.

34
Q

What are the DNA nucleotide pairings?

A

DNA nucleotide pairs purines with pyrimidines Adenine with Thymine, and Guanine with Cytosine.

mneomonic: Pure As Gold
‘CUT the PYe’

35
Q

what is alternative splicing and splice acceptor site?

A

Alternative splicing is a regulated process during gene expression that results in a single gene coding for multiple proteins. In this process, particular exons of a gene may be included within or excluded from the final, processed messenger RNA (mRNA) produced from that gene. When exons can be exised in pre-mRNA - you can assume there is a splice acceptor site

36
Q

What effect does increasing the threshold for firing action potentials have on the action potential?

A

When asked to decrease individual action potentials, you can increase the threshold for the depolarization, increase the A.R.P or limit the amount of ions present for the firing.

37
Q

What effect does temperature have on protein activity?

A

Temperature increase, causes the activity of all proteins to increase as long as their is no denaturing. But when measuring the membrane fluidity of molecule, understand that temperature is increased as a component of fluidity but not to measure the rxn rate based on temperature.

38
Q

What is a hydrophobic planar structure? Can it diffuse across a membrane freely?

A

When a substance is planar and hydrophobic or lipophilic it is most likely to be able to freely diffuse across a membrane.

39
Q

What causes the initial filtration in the glomerulus?

A

Initial filtration in the glomerulus of kidneys occurs by passive diffusion due to the pressure difference. The blood pressure forces fluid in to the Bowman’s Capsule.

40
Q

Can a enzyme alter the primary structure of a substrate? how about the shape?

A

Enzyme cannot alter primary structure of substrates. The primary structure contains the amino acid sequence of the protein. If you change the primary structure you will denature the substrate.

41
Q

Can a enzyme be found in a protein synthesized in vivo if it does not have a genetic code? why?

A

Without a corresponding codon in the genetic code, an amino acid may not take part in the process of protein translation from an mRNA transcript.

42
Q

What is a catalyst?

A

Anything with an ase is an enzyme. Enzymes are catalyst which speed up a rxn rate.

43
Q

Make sure before continuing to the next question that your answer choice you pick is the one selected on the exam

A

Make sure before continuing to the next question that your answer choice you pick is the one selected on the exam

44
Q

What does highly specific enzyme mean?

A

Highly specific means you can distinguish the activity for an enzyme compared to other enzymes because it catalyzes only specific rxns. Make sure to look at every answer choice

45
Q

what does a competitive inhibitor do?

A

Competitive inhibitor, inhibits the activity of a enzyme by competing with the substrate for the enzyme.

46
Q

Protein level in body deals with what?

A

Proteins level directly deal with the mRNA

47
Q

Is alcohol removed by cp-450

A

Alcohol is a toxin. When given in the passage that a protein is inducible like cp-450 it will tend to be more in the body naturally relating to tolerance. Read passage carefully

48
Q

Make sure before continuing to the next question that your answer choice you pick is the one selected on the exam

A

Make sure before continuing to the next question that your answer choice you pick is the one selected on the exam

49
Q

What is the major cell required for exchange in tissues?

A

Endothelial cells is the main cell in capillaries and the main cell required for exchange of nutrients such O2 in the blood and surrounding tissues

50
Q

What happens when the to the hypothalamic factor conc when adrenal cortex is damaged?

A

When the mineralcorticoid or glucocorticoids are not being delivered to the body because of a adrenal cortex damage, the hypothalamus will release more of hypothalamic factors to try to stimulate the adrenal cortex.

51
Q

what is the dihybrid cross?

A

Dihybrid crosses are used to know the probibilty of two genes in offspring from each parent

52
Q

What would the nucleotide sequence of original sequence be of a mRNA transcript of a RNA Virus?

A

mRNA transcribed from retroviral DNA is either used to synthesize viral proteins or used as RNA genome for progeny of viruses. Thus, the Sequence of nucleotide in the original viral genome will be the same as that of the transcribed mRNA.

53
Q

What is the average weight of a amino acid?

A

The average molecular weight of a amino acid is 110Da. For example a a 288 residue protein will have a avg weight of 32 kDa

54
Q

What do denaturing agents and reducing agents do in gel electrophoresis?

A

Using denaturing agents in a gel electrophoresis will disrupt the interactions between monomers of a protein, and introducing a reducing agent only affects disulfide bonds

55
Q

Convergent vs divergent evolution

A

Convergent evolution - distantly related organisms independently evolve similar traits to adapt to similar needs.

Divergent evolution - two separate species evolve differently from a common ancestor.

56
Q

what happens if GNRH isnt working?

A

Gonadotropin-releasing hormone regulates pituatary gonadotropin (leutinizing hormone and follicle stimulating hormone) secretion. If Gonadotropin - releasing hormone is not able to regulate LH and FSH, from the pituatary then the reporductive axis will remain quiescent.

57
Q

What type of enzyme is a protease

A

Protease and caspases are hydrolases

58
Q

where is a operon containing two genes in prokaryotic cell transcribed from?

A

an operon containing two genes in a prokaryotic cells is transcribed from a single promoter upstream of the first gene in the operon

59
Q

what are + ionotropic effects?

A

(+) ionictropic effects include decrease transport of calcium to the ecm, increase availability of calcium intracellularly to bind troponin and lastly increased calcium stores in sarcoplasmic reticulum

60
Q

What is Histone Acetylation?

A

Histone Acetylation usually promotes transcription by modifying chromatin structure and they are inhibited if you condense chromatin structure.

61
Q

What increases in prolonged fasting or starvation?

A

Fasting leads to glycogen breakdown and gluconeogenesis, then continued fasting (prolonged starvation or exercise) leads to production of ketone bodies via fatty acid oxidation. betaOHB is a form of ketone bodies.

62
Q

Read the graphs properly and return to the point of the data where the question is hinting or pointing towards, any other answer choice most likely may be a trap answer

A

Read the graphs properly and return to the point of the data where the question is hinting or pointing towards, any other answer choice most likely may be a trap answer

63
Q

What is Tubulin and its role in experiments?

A

Tubulin, is a cytoskeletal protein, it is stable under most conditions including hypoxia. It is used as a loading control to ascertain an equal amount of protein loading in each lane.

64
Q

what are the key enzymes of Krebs Cycle and what does succinyl co-A synthetase do?

A

Succinyl Co-A synthetase makes Succinyl Co-A into succinate in the krebs cycle and produces 2 GTP molecules as well

65
Q

What is a imprinted gene?

A

Imrpinted Gene - expressed in a parent specific manner Their expression is determined by the parent that contributed them. Imprinted genes violate the usual rule of inheritance that both alleles in a heterozygote are equally expressed.

66
Q

What terminal do amino acids get added to a polypeptide chain?

A

Assuming protein synthesis is under way when radioactive amino acids are added, infers that the N-terminal and end and probably the middle part of the protein were already sythesized before addition of radioactive amino Acids. Addition of amino acids occur at the C terminus.

67
Q

When a graph or data states that a protein is secreted only when its produced in mature form understand its graph of level of secretion may have a delay due to the fact it accomodates the time it takes for it to be in the mature form

A

When a graph or data states that a protein is secreted only when its produced in mature form understand its graph of level of secretion may have a delay due to the fact it accomodates the time it takes for it to be in the mature form

68
Q

How do RNA Viruses replicate themselves?

A

RNA Viruses require a type of transcriptase (reverse transcriptase) to replicate themselves. This is what copies viral RNA.

69
Q

Try to make a flow chart of what happens in the passage.

A

Try to make a flow chart of what happens in the passage.

70
Q

what is the equilibrium const

A

The equilibrium constant is given by Keq=Kforward/Kreverse

71
Q

Make sure to pick the answer choice you feel is the most right. GO WITH YOUR GUT ANSWER

A

Make sure to pick the answer choice you feel is the most right. GO WITH YOUR GUT ANSWER