Rickettsia, Chlamydia, Spirochetes, Ureaplasma, Mycoplasma

Question 1

Obligate intracellular organisms

Answer 1

Rickettsia
Chlamydia

Question 2

Culture media for Rickettsia

Answer 2

Yolk sac / embryonated eggs

Question 3

This is where Rickettsia multiplies inside man

Answer 3

Endothelial cells

Question 4

SPOTTED GRP:

Rocky Mountain Spotted Fever

Answer 4

R. rickettsii

Question 5

SPOTTED GRP:

Rickettsial pox (Mites)

Answer 5

R. akari

Question 6

SPOTTED GRP:

Australian/Queensland tick typhus (Ticks)

Answer 6

R. australis

Question 7

SPOTTED GRP:

Boutonneuse fever (Ticks)

Answer 7

R. conorii

Question 8

TYPHUS GRP:

Epidemic typhus
Sporadic typhus
Brill-Zinser disease

(Lice)

Answer 8

R. prowazekii

Question 9

TYPHUS GRP:

Murine typhus
Endemic typhus

(Fleas)

Answer 9

R. typhi

Question 10

Scrub typhus (Mites-Chiggers)

Answer 10

Orientia tsutsugamushi

Question 11

Q fever (Ticks-Aerosols)

Answer 11

Coxiella burnetti

Question 12

EHRLICHIOSIS

Human MONOCYTE ehrlichiosis (Ticks)

Answer 12

Ehrlichia chaffeensis

Question 13

EHRLICHIOSIS

Human GRANULOCYTE ehrlichiosis ( Ticks)

Answer 13

Ehrlichia phagocytophila
Ehrlichia ewingii

Question 14

EHRLICHIOSIS

Senetsu fever (Ticks)

Answer 14

Neorickettsia sennetsu

Question 15

2 forms of Chlamydia:

Answer 15

Reticulate body (replicative)
Elementary body (infectious)

Question 16

Detection of Elementary bodies in Chlamydia trachomatis

Answer 16

DFA: Direct Fluorescence Antibody staining

(Fluorescein-isothiocyanate conjugated monoclonal abs) FITC

Question 17

McCoy Cells for culture

Answer 17

Chlamydia

Question 18

TWAR strain
Taiwan Acute Respiratory Strain

Answer 18

Chlamydia pneumoniae

Question 19

Psittacosis/Ornithosis

Answer 19

Chlamydia psittaci

Question 20

Causative agent of:

Chlamydia, Trachoma, Lymphagranuloma venereum, Inclusion conjunctivitis

Answer 20

Chlamydia trachomatis

Question 21

Pleomorphic organisms that lack cell wall

Answer 21

Ureaplasma
Mycoplasma

Question 22

Smallest free-living organisms capable of growing in artificial media

Answer 22

Ureaplasma
Mycoplasma

Question 23

Ureaplasma and Mycoplasma requires what growth factor to survive

Answer 23

Sterols (Cholesterol)

Question 24

Primary atypical pneumonia
Walking pneumonia

Answer 24

Mycoplasma pneumoniae

Question 25

Medium for Mycoplasma pneumoniae

Answer 25

SP4 broth (yellow)

Question 26

Post abortal fever Postpartum fever

Answer 26

Mycoplasma hominis

Question 27

Fried egg appearance; cause of genital mycoplasma

Answer 27

Mycoplasma hominis

Question 28

Manganese chloride test (+) Urease (+)

Answer 28

Ureaplasma urealyticum

Question 29

Cork-screw motility

Answer 29

Spirochetes

Question 30

Axial filaments for motility

Answer 30

Spirochetes

Question 31

Slender with tight coils

Answer 31

Treponema

Question 32

Thick, few, looser coils

Answer 32

Borrelia

Question 33

Res. Borrelia but with hooked ends

Answer 33

Leptospira

Question 34

Comma, helical-shaped, tapered ends Four flagella/ each end

Answer 34

Brachyspira

Question 35

Venereal syphilis

Answer 35

Treponema pallidum subsp. pallidum

Question 36

Yaws

Answer 36

T. pallidum subsp. pertenue

Question 37

Endemic nonvenereal syphilis

Answer 37

T. pallidum subsp. endemicum

Question 38

Pinta

Answer 38

T. carateum

Question 39

Stages of syphilis: (4)

Answer 39

Primary: Chancre Secondary: Condylomata (wart-like lesions) Latent: absent symptoms Tertiary: Gummas + Neurosyphilis

Question 40

Microscope used for Treponema

Answer 40

Dark-field

Question 41

Nontreponemal tests

Answer 41

VDRL RPR

Question 42

Treponemal tests

Answer 42

FTA-ABS TP-PA

Question 43

Louse-borne epidemic relapsing fever

Answer 43

Borrelia recurrentis

Question 44

Lyme disease

Answer 44

Borrelia burgdoferi (Ixodes ticks)

Question 45

Bull's eye lesion

Answer 45

Borrelia burgdoferi Lyme disease

Question 46

Causative agent of Leptospirosis:

Answer 46

Leptospira interrogans

Question 47

Specimen for Early infection for leptospirosis

Answer 47

BLOOD

Question 48

Specimen after second week of infection of leptospirosis

Answer 48

URINE

Question 49

Media for Leptospira

Answer 49

EMJH: Ellinghausen-McCullough-Johnson-Harris Fletcher's medium