GM POS

Question 1

Coagulase: +
VP/Acetoin: +
PYR: -

Answer 1

Staph aureus

Question 2

Differentiate S. epidermidis vs S. saprophyticus

Answer 2

Novobiocin

S. epidermidis (S) >16mm
S. saprophyticus (R)

Question 3

Produces Streptolysin O and S

Answer 3

Strep pyogenes (Grp A)

Question 4

O2 labile
Subsurface hemolysis
Immunogenic

Answer 4

Streptolysin O

Question 5

O2 stable
Surface hemolysis
Nonimmunigenic

Answer 5

Streptolysin S

Question 6

BACITRACIN SENSITIVE

a. Strep pyogenes (grp A)
b. Strep agalactiae (grp B)

Answer 6

Strep pyogenes (grp A)

Question 7

MIBON positive

Answer 7

Strep pneumoniae

Question 8

Why do you need to differentiate Group D enterococcus vs non enterococcus?

Answer 8

Both are Bile esculin +

Question 9

Used to diff Enterococcus vs Nonentero

Answer 9

PYR + 6.5% NaCl

Enterococcus (+)
Nonentero (-)

Question 10

2 Spore-forming bacilli

Answer 10

Bacillus (aerobe)
Clostridium (anaerobe)

Question 11

Causes ANTHRAX, disease of wild and domestic animals (sheep, goats, horses, cattles)

Answer 11

Bacillus anthracis

Question 12

Hippurate and CAMP (+)

a. Strep pyogenes (Group A)
b. Strep agalactiae (Grp B)

Answer 12

b. Strep agalactiae (Grp B)

Question 13

Rheumatic Heart Fever
Acute Glomerulonephritis

due to Post-streptococcal sequelae

Answer 13

Strep pyogenes (grp A)

Question 14

Cause of Malignant pustule, Black eschar

Answer 14

Bacillus anthracis (cutaneous anthrax)

Question 15

Cause of Woolsorter’s disease, Rag-pickers

Answer 15

Bacillus anthracis (pulmonary anthrax)

Question 16

Primary cause of bacterial pneumonia and otitis media

Answer 16

Strep pneumoniae

Question 17

Fried rice bacillus

Answer 17

Bacillus cereus

Question 18

Causes Progressive Endophthalmitis

Answer 18

Bacillus cereus

Eye/blindness

Question 19

Why do you need to differentiate B. anthracis vs B. cereus

Answer 19

Both are Catalase and Lecithinase (+)

Question 20

differentiate B. anthracis vs B. cereus

Answer 20

Motility + Hemolysis

B. anthracis = NM, Gamma
B. cereus = M, Beta

Question 21

Foodborne pathogen with a TUMBLING motility

Answer 21

Listeria monocytogenes

Question 22

McBride medium

Answer 22

Listeria

Question 23

Umbrella-like appearance on semisolid medium

Answer 23

Listeria monocytogenes

Question 24

Produces Brown halo on Tinsdale medium

Answer 24

Corynebacterium diptheria, ulcerans, pseudotuberculosis

Question 25

UREASE (-) Corynebacterium

Answer 25

C. diptheriae

Question 26

A true pathogen that requires demonstration of toxin production

Answer 26

C. diptheriae

Question 27

Multi-drug resistant Corynebacterium

Answer 27

C. jeikeium

Question 28

Gram (+) coccobacilli, forms V shape and pallisades

Answer 28

Corynebacterium urealyticum

Question 29

The only catalase negative, GM (+), non-spore-forming rod with H2S production

Answer 29

Erysipelothrix rhusiopathiae

Question 30

Erysipeloid Erysipelas Erythrasma

Answer 30

Erysipeloid = E. rhusipathiae Erysipelas = Strep pyogenes (Grp A) Erthrasma = Corynebacterium minutissimum

Question 31

Tomato juice agar

Answer 31

Lactobacillus

Question 32

Human blood bilayer tween agar (HBT)

Answer 32

Gardnerella vaginalis

Question 33

Clue cells

Answer 33

Gardnerella vaginalis

Question 34

Whiff or Sniff test for G. vaginalis uses what reagent?

Answer 34

10% KOH

Question 35

Resembles beta-hemolytic strep and differentiated with gram stain

Answer 35

Arcanobacterium

Question 36

Causes Primary pulmonary infection res. tuberculosis

Answer 36

Nocardia

Question 37

Gram (+) Beaded appearance Urease (+) Partially acid fast Catalase (+) Strict Aerobe

Answer 37

Nocardia

Question 38

Coccobacilli in zigzag configuration

Answer 38

Rhodococcus

Question 39

Extensive hyphae on tap water agar (NNS)

Answer 39

Nocardia Nocardiopsis Streptomyces

Question 40

Lysozyme resistance

Answer 40

Nocardia Tsukamurella Rhodococcus

Question 41

Branching hyphae on tap water agar

Answer 41

Dermatophilus