Rh negative

Question 1

with deletion of the RHD gene

Answer 1

European

Question 2

No RHD, but with inherited 2 RHCE genes

Answer 2

European

Question 3

The RHD gene in African individuals that are Rh negative is called

Answer 3

RHD pseudogene (RHD ѱ) or RHD-negative allele

Question 4

An “RHD-negative allele”

Answer 4

African

Question 5

in African, sequence is identical to the RHD gene except for missense mutations in what exon?

Answer 5

exon 5 and exon 6

Question 6

African

nonsense mutation in what exon?

Answer 6

6

Question 7

in African, there are ___ base pair insertion at the intron __ and exon __ boundary

Answer 7

37 base pairs
intron 3
exon 4

Question 8

Person with this gene does not produce RhD proteins

Answer 8

RHD pseudogene (African)

Question 9

agglutination when doing IAT/HAT indicates?

Answer 9

weak D positive

Question 10

no agglutination indicates?

Answer 10

D negative

Question 11

how to test D negative vs Weak D?

Answer 11

• Incubate cells with Anti-D typing sera at 37C for 30 minutes
• Centrifuge
• Check for agglutination
• If there is no agglutination, proceed to Anti-Human Globulin Test
• Add Coomb’s reagent and centrifuge

Question 12

reagent used in testing D negative and weak D

Answer 12

Coomb’s reagent

Question 13

this is required by the AABB standards for Blood Banks and Transfusion Services for
donor units – The units should be labelled as __

Answer 13

Weak D

labelled as “Rh positive”