REVIEW PART 2

Question 1

Strengths and weaknesses of GCaMP (genetically encoded Ca indicators)

Answer 1

S: High spatial width of field
W: Moderate temporal resolution
-Rats breed in these crosses don’t get along, mother eats child, difficult to use females (menstrual cycle affects ENS)

Question 2

What is psychophysics?

Answer 2

What the animal perceives and behaviour of animal in response to stimuli

Question 3

Only good thing about PET?

Answer 3

Can be used to study binding sites for drugs and transmitters

Question 4

What radiation does PET use?

Answer 4

Gamma Rays

Question 5

Where is gephyrin NOT found?

Answer 5

Cholinergic synapses

Question 6

Rab 3

Answer 6

Targets vesicles to docking site via RIM

Question 7

Rab 5

Answer 7

Reuptake after exocytosis

Question 8

How many SNARE complexes needed for stable docking

Answer 8

3

Question 9

When is Ca2+ not required for SNARE fusion?

Answer 9

Secretion of some peptide hormones (Glucagon and Insulin)

Question 10

What unwinds SNARE complex?

Answer 10

NSF and SNAP proteins

Question 11

B end of microtubule is + or -

Answer 11

+ - the extension and shortening end

Question 12

What proteins does slow axonal transport carry?

Answer 12

Structural and cytosolic - ANT ONLY

Question 13

During insp, does thoracic pressure increase or decrease? What does this do to BP

Answer 13

Decrease

Increase BP -> increase baro -> decrease symp

Question 14

Where does the afferents of baroreceptors terminate?

Answer 14

Dorsal medial medulla or NTS

Question 15

RVLM tonically inhib or excit

Answer 15

Excit

Question 16

CVLM releases what NT

Answer 16

GABA

Question 17

Symp activity

• Regular
• Irregular
• Bursts

Answer 17

Regular - avg pressor response
Irregular - Greater
Bursts - GREATEST

Irregular and bursts both release NA and NPY
(nature of stimulus determines composition of NT released)