Review of PB and BM Morphology - Malignant

Question 1

Hematopoietic specific lineage marker?

Answer 1

CD45

Question 2

Immaturity specific markers?

Answer 2

HLA-DR (MHC-II)
CD34
TdT (lymphoid)
CD117 (myeloid)

Question 3

B lymphoid markers?

Answer 3

CD19
CD20
sCD22/cCD22
 CD10 (CALLA)
kappa/lambda Ig light chain
sIg or cIg

Question 4

T lymphoid markers?

Answer 4

CD1a (immature)
CD2
sCD3/cCD3
CD4
CD5
CD7
CD8
CD99 (immature)

Question 5

Myeloid markers?

Answer 5

CD13
CD14 (monocytic)
CD15
CD33
CD64/65 (monocytic)
Myeloperoxidase
Lysozyme

Question 6

NK cell markers?

Answer 6

CD16

CD56

Question 7

Histochemistry markers?

Answer 7

PAS (block-like in ALL)
MPO (myeloid)
NSE (monocytic)

Question 8

Morphology of burkitt leukemia blasts?

Answer 8

Intermediate sized blasts
Mature appearing lymphoid chromatin
Peripherally place nucleoli
Deeply basophillic cornflower blue cytoplasm
Often cytoplasmic vacuoles

Question 9

Burkitt leukemia flow?

Answer 9

CD45+
HLA-DR+
CD19+
CD20+
sCD22+
CD10+
sIgM+
Clonal sIgkappa
TdT-
T cell/myeloid neg

Question 10

Burkitt leukemia/lymphoma genetics?

Answer 10

t(8;14) (q24;q32) MYC with IGH
t(8;22) (q24;q11) MYC with IGL
t(2;8) p12;q24) MYC with IGK