Review Exam 1 Flashcards
A meter is the standard unit of
Length
A liter is the standard unit of
Volume
A gram is the standard unit of
Weight
Osmometry is
The measurement of the osmolality of an aqueous solutions (serum, plasma or urine)
Techniques of choice/ used most often
Freezing point depression
Serological pipettes are
Used to measure and transfer diff amounts of liquid.
Scrolling bulb can only be used with
Serological pipette
To obtain the desired amount of liquid. To deliver the states volume
The liquid in the tip of the pipette is ‘blown out’, using a bulb. (Etched or colored rings on pipette)
Molarity is defined
As the number of miles of solute per liter of solution
m / l (mol/L)
Normality is
amount of solute to the total volume of solution
n / 1 L (Eq/L)
Ways a spectrophotometer quality assurance can be used
Wavelength accuracy, stray light, linearity, bandwidth, photo metric accuracy
A solute is
Dissolving in liquid
Analytes
Biological substances
Solvent is
Liquid in which solute is dissolved
Solution is
Solute and solvent
Colligative properties is
Osmotic pressure, vapor pressure, freezing point, boiling point
Redox potential is
Measure of ability of solution to accept or donate electrons
Conductivity is
Measure of how well electricity passed through a solution
pH
Inverse log of hydrogen ion concentration
Buffers
Weak acids or bases. Stabilizes pH
Type water Used most for routine lab procedures
Type 2 water
Standard ref materials certifies by Nist are used
Primary standards (grade c). Exact concentration known.
For specimen sampling processing, in hemolysis you should notice
Pink to red. Rupture of rbc’s
For specimen sampling processing, in icterus you should notice
(From bilirubin) color is florisent red. Orange to brown. caused by liver disease
For specimen sampling processing, in lipemia you should notice
Fat in blood. Makes plasma look like milk
TD is what kind of pipette
Drain out pipette
For a high degree of accuracy use a
Volumetric pipette.
Non volumetric glasswares
Beaker and flask
Volumetric glasswares
Volumetric flask, graduated cylinder
Two types of centrifuge
Swing or fixed
Specific gravity
The density of a solution relative to water
One of the Beers Laws
Absorption of visible light by compound is directly proportional to the concentration of the compound
pH ranges
0-6.9 is acidic
7.0 Is neutral
7.1-14 is basic (alkaline)
0-14 is pH range
Name monochromators
Filters, prism, grating, slits
Formula for beers law
Ct= At/As • Cs
C t A s Stands for in the Beers law
C- concentration
t - test (unknown)
A- absorbance
s- standard ( calibrator)