Review Flashcards
Catalytic activity of enzymes depends on the presence of small molecules called…
cofactors
Cofactors are can..
execute chemical reactions that cnnot be performed by the standard set of twenty amino acids.
A apoenzyme is
enzyme without its cofactor
A haloenzyme is
a complete catalytically active enzyme
A coenzyme is…
subdivide of cofactors that are often derived from vitamins that are either tightly or loosely bound to a enzyme.
Prosthetic group..
tightly bound coenzyme
Cosubstrates..
loosely bound coenzymes that act more like substrates because they bind to the enzyme and are released from it.
Free energy
(G) a property that is a measure of useful energy, or the energy that is capable of doing work.
A reaction can take place spontaneously only if ∆G is …..
Negative
A chemical reaction of substrate (S) to form product (P) goes through a __________ that has a higher free energy than does either S or P.
transition state
The active site
a three dimensional cleft, or crevice formed by groups that come from different parts of the amino acid sequence
Induced fit
A dynamic recognition when the active site of some enzymes assumes a shape that is complementary to that of the substrate only after the substrate has been bound.
What is km or Michaelis constant equation?
km = (k2 + k3) / k1
Maximal rate is
Vmax = k2[E]T
What is the Michaelis-Menton equation?
V0 = Vmax [S] / [S] + Km
