Review 2 Flashcards

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1
Q

what is genome

A

the complete genetic material of an
organism (i.e. all DNA)

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2
Q

what is prokaryotic genome

A

single, double-stranded
DNA molecule in the form of a loop or circle.

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3
Q

what is eukaryotic genome

A

several double-stranded,
linear DNA molecules bound together with
proteins to form complexes called
chromosomes
* Each species of eukaryote has a different
number of chromosomes in the nuclei of its
cells
* E.g. human body cells (somatic cells) have 46
chromosomes (23 pairs)

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4
Q

what is diploid

A

when a cell contains two matched
sets of chromosomes

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5
Q

what is haploid

A

: when a cell has only one set of
chromosomes and is designated n
* Found in sex cells of eukaryotic organisms
* Gametes (sex cells) contain only one set of
chromosomes
* E.g. Human gametes (e.g. egg or sperm) contain
one set of 23 chromosomes
* Somatic cells in some organisms are haploid, but
this is rare (e.g. male bees, ants)

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6
Q

what is homologous chromosomes

A

the matched pairs of
chromosomes in a diploid organism

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7
Q

what is locus

A

the location at which specific nucleotide
segments are placed on a chromosome

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8
Q

what is gene

A

the functional unit of a chromosome,
which determines specific characteristics by
coding for specific proteins.

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9
Q

what is traits

A

the different forms of a characteristic.
* E.g. in humans: shape of earlobes is a
characteristic, free/attached are traits
* E.g. in humans, eye colour is a characteristic,
blue/green/brown/hazel/etc. are traits

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10
Q

what is the cell cycle

A

series of events involving cell
growth and cell division that produces two
new daughter cells.

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11
Q

what happens during interphase

A

the cell grows and DNA is
replicated (longest phase)
The cell undergoes normal processes while also
preparing for cell division.
* For a cell to go from interphase to the mitotic
phase, many internal and external conditions
must be met.
* Three stages of interphase: G1, S, and G2
The first stage of interphase
* Called G1 (or first gap) because little change is
visible.
* At biochemical level, cell is accumulating
building blocks of chromosomal DNA and
proteins, and enough energy reserves to
replicate each chromosome in the nucleus

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12
Q

What happens during Interphase: G1 phase

A

The first stage of interphase
* Called G1 (or first gap) because little change is
visible.
* At biochemical level, cell is accumulating
building blocks of chromosomal DNA and
proteins, and enough energy reserves to
replicate each chromosome in the nucleus

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13
Q

What happens during interphase: S phase

A

S phase (synthesis phase)
* DNA replication results in the formation of two
identical copies of each chromosome (called
sister chromatids) that are firmly attached by a
centromere
* Centromere is duplicated, which allows
mitotic spindle to develop
* these orchestrate the movement of
chromosomes in mitosis and helps organize
cell division

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14
Q

What happens during Interphase: G2 phase

A
  • G2 phase (second gap phase)
  • cell replenishes its energy stores and makes the
    proteins necessary for chromosome
    manipulation.
  • Cell may grow
  • Some cell organelles are duplicated, and the
    cytoskeleton is taken apart to form the mitotic
    spindle
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15
Q

what happens during mitotic phase

A

he replicated DNA and
cytoplasmic contents are separated and the
cell divides
Divides the contents of the nucleus and
cytoplasm to make two identical daughter
cells
* A process in which duplicated chromosomes
are lined up, separated, and moved to
opposite ends of the cell, and then the cell is
divided into two new identical daughter cells.

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16
Q

what happens during synthesis phase

A

DNA replication results in the formation of two
identical copies of each chromosome (called
sister chromatids) that are firmly attached by a
centromere
- centromere is duplicated, which allows mitotic spindle to develop
G2 phase (second gap phase)
* cell replenishes its energy stores and makes the
proteins necessary for chromosome
manipulation.
* Cell may grow
* Some cell organelles are duplicated, and the
cytoskeleton is taken apart to form the mitotic
spindle

17
Q

what happens during mitosis

A

the first part of the mitotic phase, which
accomplishes nuclear division
During mitosis, the cell nucleus divides
* Divided into 5 stages: prophase,
prometaphase, metaphase, anaphase, and
telophase

18
Q

what happens during cytokinesis

A

second part of the mitotic phase,
which physically separates cytoplasmic
components into two daughter cells.

19
Q

what happens during prophase

A

The chromosomes in the nucleus are made
more accessible
* nuclear envelope and ER break down
* Nucleolus disappears
* Centrosomes begin to move to opposite poles
of the cell
* sister chromatids coil more tightly and become
visible under a light microscope

20
Q

what happens during prometaphase

A

Processes begun in prophase continue
* Chromosomes connect to cytoskeleton
* Nuclear envelope disappears
* mitotic spindle continues to get longer
* Chromosomes become more condensed and
can be visually distinguished
* Each sister chromatid attaches to spindle
microtubules at the centromere

21
Q

what happens during metaphase

A

All of the chromosomes align in a plane
halfway between the two ends of the cell
* sister chromatids are still tightly attached to
each other.
* chromosomes are maximally condensed (i.e.
each is very distinct from others)

22
Q

what happens during anaphase

A

sister chromatids are split apart at the
centromere
* Each chromosome is pulled to one side of the
cell by microtubules and centrosome
* cell becomes visibly elongated

23
Q

what happens during telophase

A

Cell genetic components are packaged as two
individual daughter cells
* chromosomes reach the opposite ends of cell
and begin to decondense (unravel)
* mitotic spindles are broken down into
monomers that will be used to assemble
cytoskeleton parts for each daughter cell
* Nuclear envelopes form around chromosomes

24
Q

what happens in cytokinesis

A

second part of the mitotic phase during which
cell division is completed by the physical
separation of the cytoplasmic components
into two daughter cells
* Stages of mitosis similar for most eukaryotes
* cytokinesis is different for eukaryotes with
cell walls (i.e. plant cells)