Restriction Enzymes and metabolizing enzymes

Question 1

Restriction enzymes that break or restrict DNA polymer at the sugar phosphate backbone

Answer 1

Endonuclease

Question 2

My lease and methylated activity, binds to host specific DNA, 4-6bp sites containing methylated adenines

Answer 2

Type 1 restriction endonuclease

Question 3

Methylated and restrict (cut) DNA recognition sites are asymmetrical, cleaves at 24-26bp from the site to the 3’ end

Answer 3

Type 3 restriction endonuclease

Question 4

Cutting and methyltransferease function

Answer 4

Type 4 restriction endonuclease

Question 5

Most used in the lab, no inherent methylation activity, bind to simple diners of symmetrical DNA recognition site (palindromic), cleave DNA directly at binding site

Answer 5

Type 2 restriction endonuclease

Question 6

Metabolizing enzyme that degrades DNA from free 3’ hydroxyl or 5’ phosphate ends, not circular DNA, make stepwise deletions to modify specific ends after restriction enzymes cut

Answer 6

Exonuclease

Question 7

Metabolizing enzyme that degrades single stranded DNA FROM 3’ hydroxyl end into mono nucleotides, optimal for long ends

Answer 7

Exonuclease 1

Question 8

Metabolizing enzyme that removes 5 mononucleotides from 3’ ends of double stranded DNA, needs the presence of Mg2+ and Mn2+, has some endonuclease activity, removes from blunt ends or nicks but not overhangs

Answer 8

Exonuclease 3

Question 9

Metabolizing enzyme that digests single stranded DNA from 5 or 3 end to remove long protruding strands

Answer 9

Exonuclease 7

Question 10

Enzyme that catalyzes the formation of phosphodiester bonds

Answer 10

DNA ligase