Polymerase, Helicase, and Methyltransferase

Question 1

Main pol enzyme in bacterial replication, multi subunit holoenzyme, for leading and lagging strand synthesis

Answer 1

Polymerase 3

Question 2

Catalyzes DNA replication, active in recombination and repair of DNA

Answer 2

Polymerase 1

Question 3

Responsible for DNA repair

Answer 3

Polymerase 2

Question 4

Synthesizes polynucleotide chains without a template to the ends of DNA, in the absence of hydrogen base pairing, used in the lab to generate 3’ labeled species

Answer 4

Terminal transferase

Question 5

In DNA isolation what minimizes endonuclease and exonuclease activity by chelating cations required by nucleases for activity

Answer 5

TE Buffer (tris-HCl and EDTA) pH of 8.0

Question 6

Release of DNA through cutting and reclosing the sugar phosphate backbone

Answer 6

Helicase

Question 7

Type of Helicase that inter converts isomers or relaxes supertwisted DNA

Answer 7

Topoisomerase

Question 8

Type of Helicase that untangles DNA through double stranded breaks and separate linked rings (concatamers)

Answer 8

Gyrase

Question 9

What type of Helicase is used in anti cancer drugs to bring out about cells death by interfering with breaking and joining activity of enzymes, trapping unfinished and broken intermediates

Answer 9

Topoisomerase

Question 10

Catalyze addition of methyl groups to nitrogen bases usually A and C

Answer 10

DNA methyltransferase