Recombination and plasmids Flashcards
At what stage does crossing over occur for recombination, what 2 other ways does recombination occur in sexual reproducing organisms
Meiosis
Recombined duplexes are randomly assorted into gametes
Gametes will then merge with other gametes of the opposite parentage
The three types of asexual reproduction recombination
Conjugation
Transduction
Transformation
Recombination with filamentous bridges where genetic information moves from one cel to the other (must be touching)
Conjugation
Genetic information can be carried from F+ to F- but not from F- to F+, this demonstrates that F+ has a
Fertility factor
Transmission of DNA carried by viruses from one cell to another, usually by bacteriophages
Transduction
Basis of modern day recombinant technology, transfer of DNA without a conjugate bridge or virus,
Transformation
Prevention of transformation can be achieved with a treatment that degrades DNA
Deoxyribonuclease (DNase)
Extra chromosomal entities, usually double stranded circles
Plasmids
Plasmids carrying the genes for inactivation or circumvention of antibiotic action (RTF)
R factor
Type of plasmid that is 1-2 copies per chromosome, ie F and some R factor
Carey genes for their own transfer and propagation, self transmissible
Large plasmids
Type of plasmid that cannot carry genes to direct their own maintenance, 20 copies per chromosome, use cell division for transformation
Small plasmids