Recombination and plasmids Flashcards

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1
Q

At what stage does crossing over occur for recombination, what 2 other ways does recombination occur in sexual reproducing organisms

A

Meiosis
Recombined duplexes are randomly assorted into gametes
Gametes will then merge with other gametes of the opposite parentage

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2
Q

The three types of asexual reproduction recombination

A

Conjugation
Transduction
Transformation

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3
Q

Recombination with filamentous bridges where genetic information moves from one cel to the other (must be touching)

A

Conjugation

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4
Q

Genetic information can be carried from F+ to F- but not from F- to F+, this demonstrates that F+ has a

A

Fertility factor

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5
Q

Transmission of DNA carried by viruses from one cell to another, usually by bacteriophages

A

Transduction

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6
Q

Basis of modern day recombinant technology, transfer of DNA without a conjugate bridge or virus,

A

Transformation

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7
Q

Prevention of transformation can be achieved with a treatment that degrades DNA

A

Deoxyribonuclease (DNase)

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8
Q

Extra chromosomal entities, usually double stranded circles

A

Plasmids

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9
Q

Plasmids carrying the genes for inactivation or circumvention of antibiotic action (RTF)

A

R factor

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10
Q

Type of plasmid that is 1-2 copies per chromosome, ie F and some R factor
Carey genes for their own transfer and propagation, self transmissible

A

Large plasmids

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11
Q

Type of plasmid that cannot carry genes to direct their own maintenance, 20 copies per chromosome, use cell division for transformation

A

Small plasmids

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