Restriction endonucleases Flashcards
1
Q
Restriction endonucleases
A
Naturally occurring bacterial enzymes part of defence system against viruses
2
Q
Restriction sites
A
Restriction enzymes cut phosphodiester bonds at palindromic sequences
3
Q
Sticky ends
A
Ends that when cut, leave staggered cut in DNA which results in overhanging bases on each strand
4
Q
Blunt ends
A
DNA strand with no overhanging ends after being cut