Gel electrophoresis Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

Gel electrophoresis

A

Technique that allows DNA fragments to be sorted according to size

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Step 1

A

DNA samples loaded into small wells at the end of rectangular gel, and gel is placed into a tray with electrodes attached

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Step 2

A

Electric current turned on and DNA moves towards positive electrode

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

DNA ladder

A

Included for comparison with pieces of DNA of a known number of base pairs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Pair size

A

DNA fragments that are smaller travel further through the gel and bigger fragments travel slower and get stuck further behind in the gel

How well did you know this?
1
Not at all
2
3
4
5
Perfectly