Restriction digest

Question 1

Restriction digest

Answer 1

restriction endonucleases (aka restriction enzymes)

Question 2

What are restriction enzymes?

Answer 2

Each restriction enzyme will only cut DNA strands at recognition sites - specific bases sequences

Question 3

Cutting specifically

Answer 3

• When DNA is cut with a restriction enzyme the resulting fragements are left with either a short overhang of a single DNA called STICKY ENDS
• Or no overhanging DNA which is called a BLUNT END

Question 4

Ligase

Answer 4

• Ligase joins DNA or RNA together by creating bonds in the sugar-phosphate backbone
• Ligase is a group of enzymes that work with restriction enzymes to join together DNA, hydrogen bonds form between complementary bases of sticky ends

Question 5

Ligase with sticky ends

Answer 5

stick ends are sealed together but gaps remain

Question 6

Ligase with blunt ends

Answer 6

Ligase seal the gaps in blunt ends