Recombinant Plasmids

Question 1

Recombinant plasmids function

Answer 1

For a gene to be expressed it needs to be inserted into a living cell - such as a bacteria

• A plasmid ( small piece of circular DNA that is naturally found in bacteria) can be used as a vector to insert a gene such as the human insulin gene into bacteria

Question 2

Vector

Answer 2

an agent or vehicle used to transfer pathogens or genes between cells and organisms

Question 3

PROCESS OF CREATING A RECOMBINANT

Answer 3

1) The DNA is extracted a from cell, cut with restriction enzymes to create sticky ends

Question 4

stage 2)

Answer 4

The DNA attaches to the plasmid as there complementary bases.

Question 5

3)

Answer 5

Ligase is added to the fragments to make the joining permanent

Question 6

4)

Answer 6

plasmids that incorporate the foreign DNA = have become recombinant plasmids.
some just wont form recombinant plasmids

Question 7

5)

Answer 7

Bacterial cells are treated to make their cell walls permeable to plasmids so that they can enter the bacteria

Question 8

6)

Answer 8

Bacteria cells with recombinant plasmids = transformed bacteria