respiratory chain, oxidative phosphorylation Flashcards
1
Q
localization
A
inner membrane
2
Q
hydrogen and electron transport
A
protons pumped out from mitochondrial matrix to intermembrane space during h or electron transport
3
Q
complex 1
A
-it is a NADH+H+ dehydrogenase - using NADH+H+ as initial H donor - net result of 4 protons pumped out from matrix - two H to reduce coenzyme Q outside complex
4
Q
complex 2
A
- is a succinate dehyrogenase - one of enzymes of TCA cycle - generates net result of production of fumarate from succinate - reduction of one coenzyme Q located outside complex - no proton pumping
5
Q
complex 3 and 4
A
- contain cytochromes
6
Q
cytochrome
A
- small proteins containing haem group - 3 types: a, b and c - according to haem and how its bound
7
Q
compex 3
A
- cytochrome c-1, cytochrome bL, bH and fe-s - coenzyme q transfers electrons via fe-s to cytochrome c1 and bL - reduced cytochrome c and 4 protons
8
Q
complex 4
A
- cytochrome c oxidase - composed of cytochrom a and a3 plus two copper complexes - generates formation of water molecule , ox of cyt cs and translocation of proteins
9
Q
ATP synthase
A
- often tered complex 5 of electron transport system - uses proton motive force of translocated proteins as force - condenses adp and phosphate to form ATP
10
Q
chemiosmotic theory
A
theory of protein pumping resulting in atp formation
11
Q
inhibitors of chemiosmosis
A
- rotenine block e- flowin complex 1 - antimycin a block e- flow in complex 3 cyanide and carbon monoxide block accesss of o2 in complex 4 - oligomycin damage f0 subunit of complex 5
12
Q
uncoupling reagents
A
- lipid soluable weak acids
- h+ can dissociate from hydroxyl group of uncoupler dinitrohenol
- dissolve in membrane and carry h+
- uncouplers block ox phosphorylation by dissipating h+ electrochem gradient
13
Q
uncoupling protein
A
- thermogenin
- produced in brown adipose of newborn mammal and hibernating
- h+ carrier
-
14
Q
A
