Respiration Flashcards

1
Q

What is the equation for respiration

A

glucose + oxygen → carbon dioxide + water + energy

C6H1206 + 6 O2 → 6 CO2 + 6 H20 + 2870kJ

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2
Q

What are the 4 stages of aerobic respiration

A

-glycolysis
-the link reaction
-kerbs cycle
-electron transport chain

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3
Q

If no oxygen is available what will occur

A

Anaerobic respiration and NAD is regenerated by reduction of pyruvate to either lactic acid or ethanol

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4
Q

How is are mitochondria adapted for respiration

A

The structure of mitochondria makes them well adapted to their function
They have a large surface area due to the presence of cristae (inner folds) which enables the membrane to hold many electron transport chain proteins and ATP synthase enzymes
More active cell types can have larger mitochondria with longer and more tightly packed cristae to enable the synthesis of more ATP because they have a larger surface area
The number of mitochondria in each cell can vary depending on cell activity
Muscle cells are more active and have more mitochondria per cell than fat cells

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5
Q

Where in the mitochondria does the link reaction take place

A

Matrix

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6
Q

Where in the mitochondria does krebs take place

A

Matrix

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7
Q

Where does electron transport take place

A

On the cristae

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8
Q

Give the similarities of chloroplasts and mitochondria

A

-both have a double membrane
-large internal membrane structure
-both fluid filled
-both contain DNA and ribosomes

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9
Q

Give the differences between the chloroplasts and the mitochondria

A

-chloroplasts have grana whereas mito have cristae
-chloroplasts have stroma and mitochondria have matrix
-choloroplasts contain chlorophyll and mito don’t

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10
Q

Give the process of glycolysis

A

-activation of glucose - glucose is phosphorylated using phosphate from 2 ATP - fructose 1,6-diphosphate is produced - this has a lower Ea
- fructose 1,6-diphosphate molecules splits into 2 TP
-TP is oxidised by 2 NAD which is oxidised to 2 NADH
-TP is also dephosphorylated by removing phosphates and producing ATP
-this then produces pyruvate

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11
Q

Where does glycolysis take place

A

Cytoplasm of the cell

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12
Q

What is the net yield of glycolysis per glucose is

A

2 ATP
+2 NADH
2 pyruvate

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13
Q

What is the link reaction

A

Pyruvate undergoes oxidative decarboxylation - oxygen and carbon removed
- electrons and hydrogen from NAD become reduced to NADH
-removal of carbon dioxide coverts the pyruvate into acetate
-the acetate then combines with CoenzymeA to form acetylCoenzymeA

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14
Q

What is the net yield of the link reaction per glucose

A

2 acetyl CoenzymeA
2 NADH
2 Carbon dioxide

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15
Q

where does the krebs cycle take place

A
  • matrix of the mitochondria
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16
Q

what is the krebs cycle

A

-cycle of enzyme-controlled reactions
-main goal is to provide a continuous supply of reduced electron carriers - to be fed into the final stage of respiration

17
Q

describe the process of the krebs cycle

A

-acetylCoA (2C) combines with Oxaloacetate (4C) to produce a 6 carbon ompound called citric acid
-cirtic acid then undergoes
-a series of oxidative decarboxylation reactions - produce %C and then 4C
this removes hydrogen ions and electrons - picked up by NAD and is reduced to NADH

-after a 4C compound is produced it undergoes oxidation and substrate level phosphorylation
where FAD is reduced to FADH - gain H+
phosphate is removed from the intermediate to produce ATP when combined with ADP

-the next 4C compound then undergoes oxidation to regenerate the oxaloacetate
NAD reduced to NADH

18
Q

what are oxidative decarboxylation reactions

A

where the compound looses a molecule of CO2
and a H+ ion by reducing either NAD to NADH or FAD to FADH

19
Q

how many times does the Krebs cycle happen per molecule of glucose

A

twice as 2 pyruvates are used in glycolysis
and so 2 acetylCoA is used in krebs

20
Q

what is the net products of the krebs cycle per glucose

A
  • 4 Carbon dioxide
  • 2 FADH
  • 6 NADH
  • 2 ATP
21
Q

what is the 4th stage of aerobic respiration

A

electron transport chain

22
Q

where does the ETC take place

A

cristae of the mitochondria

23
Q

where do the electrons come from in ETC

A

the NADH and FADH produced in the previous reactions

24
Q

how is the cristae adapted for the ETC

A

-cristae/inner membrane is folded - giving a large SA

25
Q

describe the process of the ETC

A

-within the membrane there are a series of electron carrier protein complexes arranged in descending energy levels
-as electrons pass through the carrier protiens the energy released is used to move hydrogen ions from the matrix into the intermembrane space
-this movements sets up a large H+ ion conc gradient for the movement of H+ back into the matrix
-when the ETC reaches a enzyme ATP synthase the H+ ions are removed via facilitated diffusion
-each time a H+ ion passes through the enzyme it attaches a phosphate to and ADP thus producing ATP
-when the electrons reach the end of the ETC they are picked up by oxygem - terminal electron acceptor
-each oxygen atom picks up 2H+ and 2e- to produce a molecule of H20
2H+ + 2e- + 1/2 O2 = H2O
-this process is known as oxidative phosphorylation - because ATP is made and oxygen is used up
-chemiosmaotic theory

26
Q

how many ATP is produced for every NADH

A

3ATP

27
Q

how many ATP is produced per FADH

A

2 ATP

28
Q

what are the overall amounts of NADH and how many ATP does this produce

A

-2 in glycolysis
-2 in link
-6 in krebs
10 overall = 30 ATP

29
Q

what is ther overall amount of FADH and how many ATP does it produce

A

-2 in krebs
-4 ATP