Required Practicals Flashcards
Whats the purpose of the stain
Whats the purpose of the stain
Highlight objects in a cell
What is used to make the cytoplasm show up
Eosin is used to make the cytoplasm show up
Iodine is potassium iodide solution is used to …
Iodine is potassium iodide solution is used to stain starch grains in plant cells
Preparing a microscope
Preparing a microscope
Pipette a small drop of water onto the centre of the slide
Use tweezers to place a thin section of your specimen on top of the water drop
Add a drop of stain
Add the cover slip, stand the slip upright on the slide, next to the water droplet, then tilt and lower it so it covers the specimen. ( no air bubbles)
Microbiology practical
Microbiology practical
Clean hands and work area throughly before and after the experiment
Spray bench w disinfectant
Wipe w paper towels
Use a permanent marker to mark the bottom of the nutrient agar plate
Make sure lid is tight to avoid contamination
Divide plate into three equal sections and number 1, 2, 3 around the edges
Put a dot into the middle of each section
Add ur initials, the name and date if the bacteria
Osmosis required practical
Osmosis required practical
Line up 5 test tubes in a rack
Add 10cm3 of the initial 2M sucrose solution to the first test tube and 5cm3 of distilled water to the other four test tubes
Use a pipette and draw 5cm3 of the solution from the first test tube, add it to the 5cm3 distilled water in the second test tube and mix the solution throughly
You now have 10cm3 of solution thats half as concentrated as the solution in the first test tube (its 1 mole)
Repeat the process three times to create solutions of 0.5m, 0.25m and 0.125m