Required Practical 6- Effect of antimicrobial substances Flashcards

1
Q

Control experiment?

A

Well with no amylase solution (distilled water instead)

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2
Q

Why is sample incubated at 25°C

A

Because incubating at 37°C (human body temperature) could enable pathogens to grow that are harmful to humans

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3
Q

Inhibition zone

A

Clear circle around antibiotics

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4
Q

What does zone tell you?

A

Larger inhibition zone= killed more bacteria= antibiotic works better
Little/ no inhibition zone= bacteria are resistant, antibiotic not killed by it= not effective

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5
Q

What are the control variables?

A

-size of holes: use same cork borer to cut holes
-volume of enzyme: add exactly 2 drops in each well, using pipette

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6
Q

Aspetic techniques?

A

-wipe surfaces down with antibacterial wipes before & after experiment
-use Bunsen burner in work space so convection currents draw microbes away from culture
-flame wire hoop
-flame neck of bottle- prevent bacteria entering
-keep vessels containing bacteria open for minimum amount of time

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7
Q

How can you spread bacteria evenly?

A

Using sterileinoculating loop

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8
Q

How should you tape the lid on?

A

Lightly- taping the entire dish prevents oxygen entering so promotes growth of more harmful anaerobic bacteria

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9
Q

Why are agar plates upside down?

A

-clearer observation of inhibition zones due to condensation on lid- measure zones
-prevents drops of moisture formed during incubation dropping on culture

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10
Q

Why are aseptic techniques necessary?

A

-prevent contamination of cultures from environment
-Environment not contaminated by potentially hazardous microorganisms

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11
Q

Potential risk of using non-pathogenic bacteria?

A

-could mutate into disease-causing strain

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